目的:本研究的目的是探讨葡萄糖激酶(GCK)基因的相关性,葡萄糖激酶调节蛋白(GCKR)基因多态性与中国人群GDM易感性的关系.
方法:本病例对照研究纳入2018年1月15日至2019年3月31日在湖北省妇幼保健院24~28周进行产前检查的835例GDM患者和870例非糖尿病孕妇。护士接受了收集临床信息和血液样本的培训。候选单核苷酸多态性(SNP,GCKrs1799884,rs4607517,rs10278336,rs2268574,rs730497和GCKRrs780094,rs1260326)在SequenomMassarray平台上进行基因分型。统计分析包括独立样本t检验,卡方检验,采用logistic回归和单因素方差分析评估等位基因和基因型分布的差异及其与GDM几率的相关性.
结果:年龄差异有统计学意义,孕前BMI,患者的文化程度及糖尿病家族史与对照组比较差异有统计学意义(P<0.05)。在调整了这些混杂因素后,GCKrs1799884仍与GDM显著相关(P<0.05),rs4607517、rs10278336和rs2268574、rs780094和rs1260326多态性与GDM几率无显著相关性(P>0.05)。此外,rs4607517TT基因型孕妇的空腹血糖水平明显高于CC基因型(P<0.05)。
结论:GCKrs1799884突变与中国人群的GDM几率较高相关。需要进一步的更大的研究来探索GCK和GCKR多态性与GDM易感性之间的关联。
OBJECTIVE: The aim of this study was to investigate the association of glucokinase (GCK) gene, glucokinase regulatory protein (GCKR) gene polymorphisms with the susceptibility to GDM in Chinese population.
METHODS: This
case-control study included 835 GDM patients and 870 non-diabetic pregnant women who had their prenatal examinations at 24-28 gestational weeks at the Maternal and Child Health Hospital of Hubei Province from January 15, 2018 to March 31, 2019. The nurses were trained to collect clinical information and blood samples. The candidate single nucleotide polymorphism (SNPs, GCK rs1799884, rs4607517, rs10278336, rs2268574, rs730497 and GCKR rs780094, rs1260326) were genotyped on Sequenom Massarray platform. Statistical analysis including independent sample t test, chi-square test, logistic regression and one-way ANOVA were performed to evaluate the differences in allele and genotype distributions and their correlations with the odds of GDM.
RESULTS: There were statistically significant differences in age, pre-gestational BMI, education level and family history of diabetes between
case and control group (P < 0.05). After adjusting for these confounders, GCK rs1799884 was still significantly associated with GDM (P < 0.05), but there were no significant associations between rs4607517, rs10278336 and rs2268574, rs780094 and rs1260326 polymorphisms and GDM odds (P > 0.05). In addition, the pregnant women with rs4607517 TT genotype had the significantly higher fasting blood glucose level than CC genotype (P < 0.05).
CONCLUSIONS: GCK rs1799884 mutation is associated with higher GDM odds in Chinese population. Further larger studies are needed to explore the association between GCK and GCKR polymorphisms and GDM susceptibility.