背景:已在与炎症反应相关的各种疾病中研究了制瘤素M(OSM)的表达,但其在急性缺血性卒中(AIS)中的应用仍有待探索。目的:本研究的目的是评估临床上血清OSM表达与AIS各个方面之间的相关性。
方法:于2020年10月至2021年3月在重庆医科大学附属第一医院进行单中心病例对照研究。总共134例患者被纳入AIS组,34例健康个体被纳入对照组。进行体格检查并收集静脉血样本。采用酶联免疫吸附试验(ELISA)测定血清OSM。Org10172在急性中风治疗(TOAST)分类中的试验,美国国立卫生研究院卒中量表(NIHSS)评分,磁共振成像(MRI)扫描,和改良的Rankin量表(MRS)用于评估分类,病因学,严重程度,和AIS组的预后。根据敏感性进行评估以分析血清OSM表达,病因学,严重程度,预后,以及AIS的几个危险因素。回归模型,相关性,并进行敏感性试验以探讨OSM表达与AIS各方面的相关性。
结果:AIS组血清OSM表达有统计学意义(P<0.001)。所有AIS亚组均显示OSM水平升高,三个亚组反映了统计学上的显着结果。通过血清OSM水平区分AIS患者和对照组的曲线下面积为0.747(P<0.001),最佳临界值显示敏感性为58.82%,特异性为75.37%。血清OSM表达的升高与严重程度成正比,与梗死体积不成比例,在有利的结果组中升高较少。血清OSM与AIS几个危险因素的相关性在年龄上有统计学意义,低密度脂蛋白,非高密度脂蛋白,凝血酶原时间,还有收缩压.
结论:血清OSM与AIS各方面的相关表达不同。我们的发现支持了最初的假设,即OSM与人类AIS的各个方面相关。
BACKGROUND: The
expression of oncostatin M (OSM) has been studied in various diseases related to inflammatory response, but its implementation in acute ischemic stroke (AIS) remains to be explored. Objective: The objective of this
study is to assess the correlation between serum OSM
expression and various aspects of AIS in a clinical setting.
METHODS: A single-centered case-control
study was performed in the First Affiliate Hospital of Chongqing Medical University from October 2020 to March 2021. A total of 134 patients were enrolled in the AIS group and 34 healthy individuals were enrolled in the control group. Physical examinations were performed and venous blood samples were collected. Enzyme-linked immunosorbent assay (ELISA) was used to measure serum OSM.
Trial of Org 10172 in Acute Stroke Treatment (TOAST) classification, National Institutes of Health Stroke Scale (NIHSS) score, magnetic resonance imaging (MRI) scan, and modified Rankin scale (mRS) were used to assess the classification, etiology, severity, and prognosis of the AIS group. Assessments were done to analyze serum OSM expression based on sensitivity, etiology, severity, prognosis, and several risk factors of AIS. Regression models, correlation, and sensitivity tests were performed to explore the correlation of OSM expression with various aspects of AIS.
RESULTS: There was a statistically significant elevation of serum OSM expression in the AIS group (P<0.001). All AIS subgroups showed elevation in OSM level and statistically significant results were reflected in three subgroups. The area under the curve to differentiate AIS patients and control by serum OSM level was 0.747 (P<0.001), with the optimal cut-off value showing sensitivity at 58.82% and specificity at 75.37%. The elevation of serum OSM
expression was proportional with severity, not proportional to the volume of infarct, and less elevated in the favorable outcome group. Serum OSM correlation with several risk factors of AIS was statistically significant in age, low-density lipoprotein, non-high-density lipoprotein, prothrombin time, and systolic blood pressure.
CONCLUSIONS: Serum OSM was expressed differently in correlation with various aspects of AIS. Our findings supported the initial hypothesis that OSM is correlated with various aspects of AIS in humans.