河流是抗生素抗性基因(ARGs)的重要储库。然而,目前大多数研究都集中在时间和空间分布上,某些地区仍缺乏有关城乡河流ARGs种类和丰度差异的数据。鉴于此,石家庄市选择了两条农村河流和三条城市河流。在2020年12月和2021年4月,在15个采样点收集了沉积物。宏基因组测序技术用于比较沉积物中ARG的时空变化差异。结果表明:①城市河流(4776±4452)和农村河流(1043±632)分别检测到162和79个ARGs,分别。城市河流中ARG的丰度和种类高于农村河流。②磺胺的相对丰度(SAs,27%),氨基糖苷(AG,26%),和多种药物(MD,15%)ARGs在城市河流中丰度最高,而农村河流MDsARGs的相对丰度最高(65%)。总的来说,城市河流中ARG的复杂性高于农村河流。③SAs之间呈显著正相关,AGs,MD,四环素,苯酚,大环内酯类-林可胺-链脲类(MLS),β-内酰胺,和城市河流中的二氨基嘧啶ARGs(P<0.01);然而,糖肽类ARGs与各类ARGs呈显著负相关(P<0.05,P<0.01)。农村河流MDs与SAsARGs呈显著正相关(P<0.05),但氨基香豆素之间存在显著的负相关,肽,利福霉素,和磷霉素ARGs(P&lt;0.05和P&lt;0.01)。④对于城市河流的时间变化,12月和4月检测到162ARGs(4776±4452)和148ARGs(5673±5626),分别。对于农村河流的时间变化,12月和4月共检出79种(1043±632)和46种(467±183),分别。⑤RDA分析结果表明,ARGs在城乡河流中的时空分布存在差异。相关性分析表明,城市河流ARGs与工业企业数量呈显著相关,而农村河流的ARG与畜牧业产值显著相关。总的来说,本研究确定了不同河流ARGs的主要影响因素,为不同河流ARGs的风险管理提供数据支持。
Rivers are important reservoirs of antibiotic resistance genes (ARGs). However, most current studies have focused on the temporal and spatial distribution, and data on the differences in the species and abundance of ARGs between urban and rural rivers is still lacking for certain areas. In view of this, two rural rivers and three urban rivers were selected in Shijiazhuang City. In both December 2020 and April 2021, sediments were collected at 15 sampling sites. Metagenomic sequencing technology was used to compare the differences in temporal-spatial variation for ARGs in sediments. The results showed that:① 162 and 79 ARGs were detected in urban (4 776 ±4 452) and rural rivers (1 043 ±632), respectively. The abundance and species of ARGs in urban rivers were higher than those in rural rivers. ② The relative abundances of sulfonamide (SAs,27 %), aminoglycoside (AGs,26 %), and multidrug (MDs,15 %) ARGs had the highest abundance in urban rivers, whereas the relative abundance of MDs ARGs was highest in rural rivers (65 %). On the whole, the complexity of ARGs in urban rivers was higher than that in rural rivers. ③ There was a significant positive correlation between SAs, AGs, MDs, tetracycline, phenicol, macrolides-lincosamids-streptogramins (MLS), β-lactams, and diaminopyrimidine ARGs in urban rivers (P < 0.01); however, there was a significant negative correlation between glycopeptide ARGs and all types of ARGs (P < 0.05 and P < 0.01). There was a significant positive correlation between MDs and SAs ARGs in rural rivers (P < 0.05), but there was a significant negative correlation between amino aminocoumarin, peptide, rifamycin, and fosfomycin ARGs (P < 0.05 and P < 0.01). ④ For the temporal variation in urban rivers, 162 ARGs (4 776 ±4 452) and 148 ARGs (5 673 ±5 626) were detected in December and April, respectively. For the temporal variation in rural rivers, 79 species (1 043 ±632) and 46 species (467 ±183) were detected in December and April, respectively. ⑤ RDA analysis results showed that the spatial-temporal distributions of ARGs in urban and rural rivers were different. Correlation analysis showed that the ARGs in urban rivers were significantly correlated with the number of industrial enterprises, whereas the ARGs in rural rivers were significantly correlated with the output value of animal husbandry. In general, this study identified the main influencing factors for ARGs in different rivers and provided data support for ARGs risk management in different rivers.