系统性红斑狼疮(SLE)是一种慢性风湿性疾病。内皮素-1,一种血管收缩剂,属于内皮素家族,与血管相关损害有关。迄今为止,ET-1与SLE发病机制之间的关系尚不清楚。这项病例对照研究由314SLE进行,252名非SLE疾病患者和500名健康对照。ELISA法检测血清ET-1、CCN3、IL-28B水平,和ET-1基因多态性(rs5369,rs5370,rs1476046,rs2070699,rs2071942,rs2071943,rs3087459,rs4145451,rs6458155,rs9369217)进行基因分型。SLE患者ET-1水平较高,与临床、实验室特点SLE患者血清CCN3、IL-28B水平较高,ET-1水平与两种细胞因子呈正相关。rs5370、rs1476046、rs2070699、rs2071942、rs2071943、rs3087459、rs6458155和rs2070699与SLE风险相关。Rs2070699(T,TT)与SLE患者脱发有关。Rs5370(T,TT,TG),rs1476046(G,GA),rs2071942(G,GA)和rs2071943(G,GA)与SLE患者心包炎相关,脓尿和发热表现,分别。rs3087459(CC)和rs9369217(TC)与SLE患者抗SSB抗体阳性相关。在SLE患者中,Rs5369(AA)与IgG和CRP水平相关。总之,SLE患者血清ET-1水平升高可能是潜在的疾病标志物,其基因多态性与SLE易感性有关。
Systemic lupus erythematosus (SLE) is a chronic rheumatic disorder. Endothelin-1, a vasoconstrictor, belongs to the endothelin family and is associated with vascular-related damages. To date, association between ET-1 and pathogenesis of SLE remains unclear. This case-control
study was carried out by 314 SLE, 252 non-SLE diseases patients and 500 healthy controls. Serum ET-1, CCN3, IL-28B levels were detected by ELISA, and ET-1 gene polymorphisms (rs5369, rs5370, rs1476046, rs2070699, rs2071942, rs2071943, rs3087459, rs4145451, rs6458155, rs9369217) were genotyped with Kompetitive Allele-Specific PCR. SLE patients had high levels of ET-1, which were correlated with some clinical, laboratory features. Serum CCN3, IL-28B levels were higher in SLE patients, and ET-1 levels were positively correlated with the two cytokines. Rs5370, rs1476046, rs2070699, rs2071942, rs2071943, rs3087459, rs6458155 and rs2070699 were associated with SLE risk. Rs2070699 (T, TT) was related to SLE patients with alopecia. Rs5370 (T, TT, TG), rs1476046 (G,GA), rs2071942 (G,GA) and rs2071943 (G,GA) were associated with SLE patients with pericarditis, pyuria and fever manifestation, respectively. Rs3087459 (CC) and rs9369217 (TC) were related to SLE patients with positive anti-SSB antibody. Rs5369 (AA) was associated with IgG and CRP levels in SLE patients. In conclusion, elevated serum ET-1 in SLE patients may be a potential disease marker, and its gene polymorphisms were related to SLE susceptibility.