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Abstract：
OBJECTIVE: This study aimed to evaluate the potential of Endothelin-1 (ET-1), a peptide derived from vascular endothelial cells, as a biomarker for diagnosing peri-implant diseases.
METHODS: A cohort of 29 patients with a total of 76 implants was included in this study and subsequently divided into three groups based on peri-implant clinical parameters and radiographic examination: healthy (peri-implant health) (n = 29), mucositis (n = 22), and peri-implantitis (n = 25) groups. The levels of ET-1 (ρg/site) and interleukin (IL)-1β (ρg/site) in peri-implant sulcus fluid (PISF) samples were determined using enzyme immunoassay. Statistical analyses were conducted using Kruskal-Wallis and Steel-Dwass tests. Logistic regression and receiver operating characteristic (ROC) curve analyses were performed to evaluate the diagnostic performance of the biomarkers.
RESULTS: ET-1 levels were significantly elevated in the peri-implantitis group compared to those in the healthy group, and were highest in the peri-implant mucositis group. Additionally, IL-1β levels were significantly higher in the peri-implantitis group than those in the healthy group. ROC curve analysis indicated that ET-1 exhibited superior area under the curve values, sensitivity, and specificity compared to those of IL-1β.
CONCLUSIONS: Our findings suggest that the presence of ET-1 in PISF plays a role in peri-implant diseases. Its significantly increased expression in peri-implant mucositis indicates its potential for enabling earlier and more accurate assessments of peri-implant inflammation when combined with conventional examination methods.
METHODS: A cohort of 29 patients with a total of 76 implants was included in this study and subsequently divided into three groups based on peri-implant clinical parameters and radiographic examination: healthy (peri-implant health) (n = 29), mucositis (n = 22), and peri-implantitis (n = 25) groups. The levels of ET-1 (ρg/site) and interleukin (IL)-1β (ρg/site) in peri-implant sulcus fluid (PISF) samples were determined using enzyme immunoassay. Statistical analyses were conducted using Kruskal-Wallis and Steel-Dwass tests. Logistic regression and receiver operating characteristic (ROC) curve analyses were performed to evaluate the diagnostic performance of the biomarkers.
RESULTS: ET-1 levels were significantly elevated in the peri-implantitis group compared to those in the healthy group, and were highest in the peri-implant mucositis group. Additionally, IL-1β levels were significantly higher in the peri-implantitis group than those in the healthy group. ROC curve analysis indicated that ET-1 exhibited superior area under the curve values, sensitivity, and specificity compared to those of IL-1β.
CONCLUSIONS: Our findings suggest that the presence of ET-1 in PISF plays a role in peri-implant diseases. Its significantly increased expression in peri-implant mucositis indicates its potential for enabling earlier and more accurate assessments of peri-implant inflammation when combined with conventional examination methods.
摘要：
目的：本研究旨在评估内皮素-1（ET-1）的潜力，一种来自血管内皮细胞的肽，作为诊断植入物周围疾病的生物标志物。
方法：本研究包括29名患者，共76个植入物，随后根据植入物周围临床参数和影像学检查分为三组：健康（植入物周围健康）（n=29），粘膜炎（n=22），和种植体周围炎（n=25）组。使用酶免疫测定法测定植入物周围沟液（PISF）样品中ET-1（ρg/位点）和白介素（IL）-1β（ρg/位点）的水平。使用Kruskal-Wallis和Steel-Dwass测试进行统计分析。进行Logistic回归和受试者工作特征（ROC）曲线分析以评估生物标志物的诊断性能。
结果：与健康组相比，种植体周围炎组的ET-1水平显着升高，在种植体周围黏膜炎组中最高。此外,种植体周围炎组IL-1β水平明显高于健康组。ROC曲线分析显示ET-1曲线下面积较好,灵敏度,和特异性与IL-1β相比。
结论：我们的研究结果表明，PISF中ET-1的存在在种植体周围疾病中起作用。它在种植体周围粘膜炎中的表达显着增加，表明当与常规检查方法结合时，它有可能更早，更准确地评估种植体周围炎症。
方法：本研究包括29名患者，共76个植入物，随后根据植入物周围临床参数和影像学检查分为三组：健康（植入物周围健康）（n=29），粘膜炎（n=22），和种植体周围炎（n=25）组。使用酶免疫测定法测定植入物周围沟液（PISF）样品中ET-1（ρg/位点）和白介素（IL）-1β（ρg/位点）的水平。使用Kruskal-Wallis和Steel-Dwass测试进行统计分析。进行Logistic回归和受试者工作特征（ROC）曲线分析以评估生物标志物的诊断性能。
结果：与健康组相比，种植体周围炎组的ET-1水平显着升高，在种植体周围黏膜炎组中最高。此外,种植体周围炎组IL-1β水平明显高于健康组。ROC曲线分析显示ET-1曲线下面积较好,灵敏度,和特异性与IL-1β相比。
结论：我们的研究结果表明，PISF中ET-1的存在在种植体周围疾病中起作用。它在种植体周围粘膜炎中的表达显着增加，表明当与常规检查方法结合时，它有可能更早，更准确地评估种植体周围炎症。
