纹状体棒状杆菌是革兰氏阳性细菌,其为直的或略微弯曲的并且不形成孢子。虽然它最初被认为是人类皮肤正常微生物组的一部分,越来越多的研究将其确定为各种慢性疾病的病因,菌血症,和呼吸道感染。然而,尽管它作为病原体越来越重要,病原体种群的遗传特征,如基因组特征和差异,病原体携带的抗性基因和毒力因子的类型及其在人群中的分布知之甚少。为了解决这些知识差距,我们对从不同组织和地理位置分离的314株纹状体梭菌进行了全基因组分析。我们的分析表明纹状体梭菌具有开放的泛基因组,由5692个基因家族组成,包括1845个核心基因家族,2362个附属基因家族,和1485个独特的基因家族。我们还发现纹状体梭菌在不同来源之间表现出高度的多样性,但是从皮肤组织中分离的菌株更保守。此外,通过将菌株与耐药基因数据库(CARD)和病原体毒力因子数据库(VFDB)进行比较,我们鉴定了53个耐药基因和42个毒力因子,分别。我们发现这些基因和因子广泛分布于纹状体,77.7%的菌株携带2个或2个以上的抗性基因,对氨基糖苷类表现出初级抗性,四环素,林可霉素,大环内酯类,和链霉素.毒力因子主要与宿主内的病原体存活有关,铁吸收,pili,和早期生物膜形成。总之,我们的研究提供了对人口多样性的见解,抗性基因,和C的毒力因子。纹状体来自不同的来源。我们的发现可以为未来的诊断研究和临床实践提供信息,预防,以及纹状体梭菌相关疾病的治疗。
Corynebacterium striatum is a Gram-positive bacterium that is straight or slightly curved and non-spore-forming. Although it was originally believed to be a part of the normal microbiome of human skin, a growing number of studies have identified it as a cause of various chronic diseases, bacteremia, and respiratory infections. However, despite its increasing importance as a pathogen, the genetic characteristics of the pathogen population, such as genomic characteristics and differences, the types of resistance genes and virulence factors carried by the pathogen and their distribution in the population are poorly understood. To address these knowledge gaps, we conducted a pan-genomic analysis of 314 strains of C. striatum isolated from various tissues and geographic locations. Our analysis revealed that C. striatum has an open pan-genome, comprising 5692 gene families, including 1845 core gene families, 2362 accessory gene families, and 1485 unique gene families. We also found that C. striatum exhibits a high degree of diversity across different sources, but strains isolated from skin tissue are more conserved. Furthermore, we identified 53 drug resistance genes and 42 virulence factors by comparing the strains to the drug resistance gene database (CARD) and the pathogen virulence factor database (VFDB), respectively. We found that these genes and factors are widely distributed among C. striatum, with 77.7% of strains carrying 2 or more resistance genes and displaying primary resistance to aminoglycosides, tetracyclines, lincomycin, macrolides, and streptomycin. The virulence factors are primarily associated with pathogen survival within the host, iron uptake, pili, and early biofilm formation. In summary, our study provides insights into the population diversity, resistance genes, and virulence factors ofC. striatum from different sources. Our findings could inform future research and clinical practices in the diagnosis, prevention, and treatment of C. striatum-associated diseases.