背景:使用微生物生物合成金属纳米颗粒是一种神话般的新兴的生态友好科学,具有明确的尺寸,形状和受控的单分散性。铜纳米颗粒,在其他金属颗粒中,由于它们在电子产品中的应用,引起了越来越多的关注,光学,催化作用,和抗菌剂。
结果:这项研究解释了来自土壤菌株的铜纳米颗粒的生物合成和表征,NialliaculansG9和拟杆菌。S4c采用环保方法。这些菌株提供了铜离子的最大还原和最大合成CuNPs。CuNPs的生物形成已经通过紫外-可见吸收光谱进行了表征,X射线衍射,傅里叶变换红外光谱,X射线分析和透射电子显微镜分析。使用紫外可见光谱扫描,合成的CuNPsSPR光谱在λ304和308nm处显示出最大吸收峰。所产生的CuNPs的TEM研究揭示了通过G9菌株的尺寸范围为13-100nm的球形/六方纳米颗粒和通过S4c菌株的尺寸范围为5-40nm的球形纳米颗粒的发展。还证实了CuONPs的官能团和化学组成。研究了生物合成的CuNPs对某些人类病原体的抗微生物活性。从G9菌株产生的CuNPs对白色念珠菌ATCC10,231的活性最高,对铜绿假单胞菌ATCC9027的活性最低。来自S4c菌株的CuNPs对大肠杆菌ATCC10,231的活性最高,对肺炎克雷伯菌ATCC13,883的活性最低。
结论:目前的工作集中在增加两个分离株的CuNPs产量,NialliaculansG9和拟杆菌。S4c,然后被描述为旁边的。所使用的分析和化学组成技术验证了G9和S4c生物合成的纳米铜中CuONPs的存在。与G9菌株相比,S4c的CuNPs更小,形状更多样,根据TEM图像。在抗菌活性方面,发现来自G9和S4c的生物合成的CuNPs分别对白色念珠菌ATCC10,231和大肠杆菌ATCC10,231更有效。
BACKGROUND: Biosynthesis of metallic nanoparticles using microorganisms are a fabulous and emerging eco-friendly science with well-defined sizes, shapes and controlled monodispersity.
Copper nanoparticles, among other metal particles, have sparked increased attention due to their applications in electronics, optics, catalysis, and antimicrobial agents.
RESULTS: This investigation explains the biosynthesis and characterization of
copper nanoparticles from soil strains, Niallia circulans G9 and Paenibacillus sp. S4c by an eco-friendly method. The maximum reduction of
copper ions and maximum synthesis CuNPs was provided by these strains. Biogenic formation of CuNPs have been characterized by UV-visible absorption spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, X-ray analysis and transmission electron microscopy analysis. Using UV-visible spectrum scanning, the synthesised CuNPs\' SPR spectra showed maximum absorption peaks at λ304&308 nm. TEM investigation of the produced CuNPs revealed the development of spherical/hexagonal nanoparticles with a size range of 13-100 nm by the G9 strain and spherical nanoparticles with a size range of 5-40 nm by the S4c strain. Functional groups and chemical composition of CuONPs were also confirmed. The antimicrobial activity of the biosynthesized CuNPs were investigated against some human pathogens. CuNPs produced from the G9 strain had the highest activity against Candida albicans ATCC 10,231 and the lowest against Pseudomonas aeruginosa ATCC 9027. CuNPs from the S4c strain demonstrated the highest activity against Escherichia coli ATCC 10,231 and the lowest activity against Klebsiella pneumonia ATCC 13,883.
CONCLUSIONS: The present work focused on increasing the CuNPs production by two isolates, Niallia circulans G9 and Paenibacillus sp. S4c, which were then characterized alongside. The used analytics and chemical composition techniques validated the existence of CuONPs in the G9 and S4c biosynthesized nano cupper. CuNPs of S4c are smaller and have a more varied shape than those of G9 strain, according to TEM images. In terms of antibacterial activity, the biosynthesized CuNPs from G9 and S4c were found to be more effective against Candida albicans ATCC 10,231 and E. coli ATCC 10,231, respectively.