PDF(Pubmed)
Abstract：
According to an estimate, 30% to 40%, of global fruit are wasted, leading to post harvest losses and contributing to economic losses ranging from $10 to $100 billion worldwide. Among, all fruits the discarded portion of oranges is around 20%. A novel and value addition approach to utilize the orange peels is in nanoscience. In the present study, a synthesis approach was conducted to prepare the metallic nanoparticles (copper and silver); by utilizing food waste (Citrus plant peels) as bioactive reductants. In addition, the Citrus sinensis extracts showed the reducing activity against metallic salts copper chloride and silver nitrate to form Cu-NPs (copper nanoparticles) and Ag-NPs (Silver nanoparticles). The in vitro potential of both types of prepared nanoparticles was examined against plant pathogenic bacteria Erwinia carotovora (Pectobacterium carotovorum) and pathogens effect on human health Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Moreover, the in vivo antagonistic potential of both types of prepared nanoparticles was examined by their interaction with against plant (potato slices). Furthermore, additional antipathogenic (antiviral and antifungal) properties were also examined. The statistical analysis was done to explain the level of significance and antipathogenic effectiveness among synthesized Ag-NPs and Cu-NPs. The surface morphology, elemental description and size of particles were analyzed by scanning electron microscopy, transmission electron microscopy, energy-dispersive spectroscopy and zeta sizer (in addition polydispersity index and zeta potential). The justification for the preparation of particles was done by UV-Vis Spectroscopy (excitation peaks at 339 nm for copper and 415 nm for silver) and crystalline nature was observed by X-ray diffraction. Hence, the prepared particles are quite effective against soft rot pathogens in plants and can also be used effectively in some other multifunctional applications such as bioactive sport wear, surgical gowns, bioactive bandages and wrist or knee compression bandages, etc.
摘要：
根据估计,30%到40%,全球的水果被浪费了，导致收获后损失，并在全球范围内造成100至1000亿美元的经济损失。其中,所有水果中被丢弃的橙子的比例约为20%。利用橙皮的一种新颖且增值的方法是纳米科学。在本研究中,进行了一种合成方法来制备金属纳米颗粒(铜和银);通过利用食物废物(柑橘植物皮)作为生物活性还原剂。此外,柑橘提取物对金属盐氯化铜和硝酸银具有还原活性，形成Cu-NP（铜纳米颗粒）和Ag-NP（银纳米颗粒）。研究了两种制备的纳米颗粒的体外潜力，以对抗植物病原菌欧文氏菌（Pectobacteriumcarotovorum）和病原体对人类健康大肠杆菌的影响（E。大肠杆菌)和金黄色葡萄球菌(S.金黄色葡萄球菌）。此外，通过与植物（马铃薯切片）的相互作用来检查两种类型的纳米颗粒的体内拮抗潜力。此外，还检查了其他抗致病性（抗病毒和抗真菌）特性。进行统计分析以解释合成的Ag-NP和Cu-NP之间的显著性水平和抗致病性有效性。表面形貌,通过扫描电子显微镜分析了颗粒的元素描述和尺寸，透射电子显微镜,能量色散光谱和zetasizer（此外，多分散指数和zeta电位）。通过UV-Vis光谱法(铜在339nm处的激发峰和银在415nm处的激发峰)进行颗粒制备的理由,并且通过X射线衍射观察结晶性质。因此，制备的颗粒对植物中的软腐病病原体非常有效，也可以有效地用于一些其他多功能应用，如生物活性运动服，手术礼服,生物活性绷带和手腕或膝盖压缩绷带，等。
