硫酸盐还原菌厌氧降解褐煤过程中中间液相产物在硫化氢形成过程中的变化规律和转化规律对现有的煤矿硫化氢气体成因理论具有重要的补充和完善作用。在本文中,采用气相色谱法和气相色谱-质谱法对硫酸盐还原菌厌氧降解褐煤过程中产生的H2S气体和关键中间液相产物进行了检测和分析。结果表明,硫酸盐还原菌降解褐煤生产硫化氢的过程大致可分为四个阶段:缓慢生产阶段,快速生长阶段,稳定生产阶段,和轻微的下降阶段。在这个反应体系中,SO42-浓度呈下降趋势,pH值呈增加趋势,ORP值随时间下降后略有上升。在实验过程中检测到十种挥发性成分:直链烷烃,支链烷烃,酒精,醛类,酮,烯烃,胺,脂质,酸和酚。中间液相产物中的关键组分为直链烷烃,直链烷烃,酸,酒精,酚类和胺类。PAHs,烷烃,和酚类物质与H2S的产生密切相关,而酰胺刺激氮的产生。该过程分为三个阶段:水解阶段,H2S气体生产阶段,和衰变阶段。液相中间体在煤矿BSR硫化氢的形成过程和煤矿H2S的成因机理中起着重要作用。
The changes and transformation laws of intermediate liquid-phase products during the anaerobic degradation of lignite by sulfate-reducing bacteria in the formation of hydrogen sulfide play an important role in supplementing and improving the existing theories on the genesis of hydrogen sulfide gas in coal mines. In this paper, H2S gas and key intermediate liquid-phase products produced during the anaerobic degradation of lignite by sulfate-reducing bacteria were detected and analyzed by gas chromatography and gas chromatography-mass spectrometry. The results showed that the process of hydrogen sulfide production from lignite degradation by sulfate-reducing bacteria can be roughly divided into four stages: slow production phase, rapid growth phase, steady production phase, and slight decline phase. In this reaction system, the SO42- concentration showed a decreasing trend, the pH value showed an increasing trend, and the ORP value decreased and then slightly increased with time. Ten volatile component types were detected during the experiment: straight-chain alkanes, branched-chain alkanes, alcohols, aldehydes, ketones, olefins, amines, lipids, acids and phenols. The key components in the intermediate liquid phase products were straight chain alkanes, straight chain alkanes, acids, alcohols, phenols and amines. PAHs, alkanes, and phenols are closely related to H2S production, while amides stimulate nitrogen production. The process is divided into three stages: hydrolysis stage, H2S gas production stage, and decay stage. Liquid-phase intermediates play an important role in the formation process of coal mine BSR hydrogen sulfide and the mechanism of coal mine H2S genesis.