背景:重度抑郁症(MDD)是一种常见且使人衰弱的精神疾病,其特征是持续的悲伤感,绝望,对日常活动缺乏兴趣。这项研究的目的是研究血液中巨噬细胞炎性蛋白-1β(MIP-1β)和巨噬细胞化学引诱蛋白-2(MCP-2)的水平与健康对照相比是否与MDD的病理生理和发展有关(HC)。
方法:本病例对照研究涉及50例MDD患者和38例HCs。我们进行了全面评估以匹配年龄,性别,BMI,和群体之间的社会人口统计特征。这项研究排除了慢性感染的参与者,炎症性疾病,共存的精神疾病,肝脏和肾脏疾病的历史,和服用抗精神病药物的人。专业的精神科医生诊断了MDD患者,并根据《精神障碍诊断和统计手册-5》(DSM-5)标准评估了HCs。使用汉密尔顿抑郁(Ham-D)评定量表评估抑郁的严重程度。使用市售的酶联免疫吸附测定(ELISA)试剂盒定量血清MIP-1β和MCP-2水平。
结果:结果表明,MDD患者的血清MIP-1β水平(207.73±24.24pg/ml)高于HC(58.77±9.14pg/ml)。这种浓度差异与疾病症状的严重程度呈正相关(r=0.451;p<0.001)。同样,与对照组相比,患者的MCP-2水平升高(143.61±19.92vs.56.84±4.02pg/ml;p=0.003),与Ham-D评分呈正相关(r=0.373;p=0.004)。
结论:根据这项研究,MIP-1β和MCP-2水平升高可能与MDD的病理生理和发展有关。这些升高的血清MIP-1β和MCP-2水平可用作MDD的风险评估工具。目前的发现敦促进一步研究和开发抑郁症的治疗和诊断方法。
BACKGROUND: Major depressive disorder (MDD) is a common and debilitating mental illness characterized by persistent feelings of sadness, hopelessness, and a lack of interest in daily activities. The objective of this study was to investigate whether levels of macrophage inflammatory protein-1β (MIP-1β) and macrophage chemoattractant protein-2 (MCP-2) in the blood were associated with the pathophysiology and development of MDD compared to healthy controls (HCs).
METHODS: This
case-control study was conducted involving 50 MDD patients and 38 HCs. We performed a comprehensive assessment to match age, sex, BMI, and socio-demographic profile between the groups. The study excluded participants with chronic infection, inflammatory diseases, coexisting psychiatric disorder, history of liver and kidney diseases, and individuals who are under antipsychotic medications. A professional psychiatrist diagnosed MDD patients and evaluated HCs based on the Diagnostic and Statistical Manual of Mental Disorders-5 (DSM-5) criteria. The severity of depression was assessed using the Hamilton Depression (Ham-D) rating scale. Commercially available enzyme-linked immunosorbent assay (ELISA) kits were used to quantify the serum MIP-1β and MCP-2 levels.
RESULTS: The results indicated elevated serum MIP-1β levels (207.73±24.24 pg/ml) in MDD patients compared to HCs (58.77±9.14 pg/ml). This difference in concentration is positively correlated with severity of disease symptoms (r = 0.451; p<0.001). Similarly, the levels of MCP-2 were found to be elevated in patients compared to controls (143.61±19.92 vs. 56.84±4.02 pg/ml; p = 0.003), with a positive correlation with the Ham-D scores (r = 0.373; p = 0.004).
CONCLUSIONS: According to this study, elevated levels of MIP-1β and MCP-2 may be associated with the pathophysiology and development of MDD. These increased serum MIP-1β and MCP-2 levels could be used as risk assessment tools for MDD. The present findings urge further research and the development of therapeutic and diagnostic approaches for depression.