背景:淋巴瘤可能发生在各种类型,比如淋巴瘤,淋巴肉瘤,淋巴细胞白血病,浆细胞样白血病.淋巴瘤,定义为淋巴组织的恶性肿瘤,已在许多鱼类家族中发现,包括伊社会科和沙门氏菌科。然而,淋巴瘤的发生在鲤科动物中很少见。本研究最终诊断为眼部和睾丸T细胞淋巴瘤是基于临床体征,形态学,在宏观和微观检查中肿瘤块的质地。此外,组织病理学和免疫组织化学结果与T细胞淋巴瘤的特征相对应。
方法:2020年10月,一只2岁的雌雄同体锦缎鱼(CyprinuscarpioLinnaeus1758)眼肿块大,右眼严重眼球突出,被转诊到观赏鱼诊所。在麻醉下,进行了摘除。右眼摘除后57天,发现左眼眼球突出症。手术后221天,鱼被发现死了。尸检时,发现了一个附着在左睾丸上的大软组织块。肝脏表面也有白色小结节。组织病理学显示高细胞性眼部肿块,结缔组织很少。这些切片还显示了多灶性出血,圆形到卵形肿瘤细胞,轻度至中度anisokaryosis和anisocytosis,和有丝分裂图。在睾丸肿块内的血管中发现了嗜碱性肿瘤细胞,提高了系统性蔓延的可能性。肝脏显示出微观转移,其形态与眼部和睾丸肿瘤相似。浸润左眼和右眼的肿瘤细胞以及睾丸肿块的CD3免疫组织化学阳性,但CD20阴性。根据组织病理学和免疫组织化学发现,肿块被诊断为T细胞淋巴瘤。
结论:本病例报告提供了第一个临床证据,组织病理学,形态学,以及在伊朗雌雄同体的鲤鱼(Cyprinuscarpio)中的眼部和睾丸T细胞淋巴瘤的免疫组织化学发现。
BACKGROUND: Lymphatic neoplasia may occur in various types, such as lymphoma, lymphosarcoma, lympholeukemia, and plasmacytoid leukemia. Lymphoma, defined as a malignant tumour of lymphoid tissue, has been found in a number of fish families including Esocidae and Salmonidae. However, the occurrence of lymphoma is rare in those belonging to the Cyprinidae. A final diagnosis of ocular and testicular T-cell lymphoma in the present study was based on the clinical signs, morphology, and texture of the tumour masses in the macroscopic and microscopic examinations. In addition, histopathological and immunohistochemical findings corresponded to T-cell lymphoma characteristics.
METHODS: A 2-year-old hermaphrodite koi carp (Cyprinus carpio Linnaeus 1758) with a large ocular mass and severe exophthalmia in the right eye was referred to the Ornamental Fish Clinic in October 2020. Under anesthesia, enucleation was performed. 57 days after enucleation of the right eye, exophthalmia in the left eye was discovered. 221 days after surgery, the fish was discovered to be dead. At necropsy, a large soft tissue mass attached to the left testis was discovered. There were also small whitish nodules on the surface of the liver. Histopathology revealed a hypercellular ocular mass with scant connective tissue. The sections also revealed multifocal hemorrhages, round to ovoid neoplastic cells, mild-to-moderate anisokaryosis and anisocytosis, and mitotic figures. Basophilic neoplastic cells were found in blood vessels within the testicular mass, raising the possibility of systemic spread. The liver showed microscopic metastasis with morphologic similarities to the ocular and testicular tumors. The neoplastic cells infiltrating the left and right eyes as well as the testicular mass were immunohistochemically positive for CD3 but negative for CD20. The masses were diagnosed as T-cell lymphoma based on histopathological and immunohistochemical findings.
CONCLUSIONS: This
case report provides the first evidence of clinical, histopathological, morphological, and immunohistochemical findings of an ocular and testicular T-cell lymphoma in a hermaphrodite koi carp (Cyprinus carpio) in Iran.