Abstract：
This paper evaluates the coexistence risks of triphenyltin (TPT) and norfloxacin (NOR) to aquatic organisms in the aquatic environment. Carp (Cyprinus carpio) was used as the test organism, the control and exposure groups (1 μg/L TPT), 1 mg/L (NOR), 1 μg/LTPT+1 mg/LNOR (TPT_NOR)) were set up according to the environmental concentration in the severely polluted area for 42 days. The single/combined toxic effects of TPT and NOR on aquatic organisms were evaluated by analyzing carp brain transcriptome sequencing, gut microbiota structure, and detection of biochemical indicators and RT-qPCR. Our results show that TPT and NOR induce lipid metabolism disorder in carp brain tissue, affecting the metabolism of cytochrome P450 to exogenous substances, and NOR also induces immunosuppression in carp. Long-term exposure to TPT combined with NOR amplifies the monotoxicity of TPT or NOR on lipid metabolism and immunosuppression in carp, induces immune dysfunction in brain tissue and changes in gut microbiota structure. However, TPT_NOR has no obvious neurotoxicity on the brain, but it can inhibit the level of intestinal MDA. This highlights that co-exposure of TPT and NOR amplifies metabolic disorders and immunosuppressive functions in carp.
摘要：
本文评价了水环境中三苯基锡(TPT)和诺氟沙星(NOR)对水生生物的共存风险。鲤鱼（Cyprinuscarpio）被用作测试生物，对照组和暴露组（1μg/LTPT），1mg/L（NOR），根据严重污染区的环境浓度设置1μg/LTPT1mg/LNOR（TPT_NOR）），持续42天。通过分析鲤鱼脑转录组测序，评估TPT和NOR对水生生物的单一/联合毒性作用，肠道菌群结构，生化指标和RT-qPCR检测。我们的结果表明，TPT和NOR诱导鲤鱼脑组织脂质代谢紊乱，影响细胞色素P450对外源物质的代谢，和NOR也在鲤鱼中诱导免疫抑制。TPT联合NOR长期暴露会放大TPT或NOR对鲤鱼脂质代谢和免疫抑制的单毒性,诱导脑组织免疫功能障碍和肠道菌群结构的变化。然而,TPT_NOR对脑无明显神经毒性,但是它可以抑制肠道MDA的水平。这突出表明TPT和NOR的共同暴露会放大鲤鱼的代谢紊乱和免疫抑制功能。
