背景:药物相关的颌骨坏死(MRONJ)是一种主要发生在接受或先前接受双膦酸盐治疗的患者中的疾病,特别是在存在风险因素的情况下,如拔牙(TE)。
目的:本研究旨在评估硒(SEL)在MRONJ动物模型中预防颌骨坏死的作用。
本研究是在48只Wistar大鼠的样品中使用TE模型的纵向体内动物研究。
方法:预测变量是SEL暴露,SEL曝光的定时,和唑来膦酸(ZOL)暴露。将动物随机分配到4个治疗组(每组n=12):1)生理盐水(阴性对照),2)ZOL(阳性对照),3)SELpreop+ZOL,和4)ZOL+SELposop。给动物施用盐水(阴性对照)或ZOL(0.06mg/kg,腹膜内)每周一次,共5周。所有大鼠在第五周末接受TE。SEL(0.3mg/kg,腹膜内)在TE之前每天一次给予SELpreopZOL组15天,在TE之后给予ZOLSELpostop组。在第9周结束时处死所有动物。
方法:主要结果变量是新骨面积,坏死骨区域,纤维化,新的结缔组织形成,组织病理学分析中的炎性细胞浸润,以及免疫组织化学分析中的血管生成和成骨细胞百分比。
■没有。
方法:使用Kruskal-Wallis检验进行统计分析,接下来是事后Bonferroni校正的Mann-WhitneyU测试,显著性水平为P≤0.05。
结果:ZOL+SELpostop组(3.00分)的新骨面积高于生理盐水组(0.58±1.08分,P<.001)和ZOL组(0.82±1.40分,P=.001),而ZOL+SELpostop组坏死骨面积(0.08±0.29分)低于ZOL组(2.82±0.40分,P<.001)和SELpreop+ZOL组(1.67±0.89分,P=.007)。ZOL+SELposstop组成骨细胞百分比(18.73%)高于生理盐水组(8.63%,P<.001)和ZOL组(0.07%,P<.001),SELpreop+ZOL组(18.49%)也高于ZOL组(0.07%,P<.001)。
结论:结论SEL阻止MRONJ,术后SEL显示出更大的预防效果。鉴于这些发现,我们假设SEL暴露可能会降低MRONJ的风险。
BACKGROUND: Medication-related osteonecrosis of the jaw (MRONJ) is a condition that can occur primarily in patients undergoing or have previously undergone therapy with bisphosphonates, particularly in the presence of risk factors, such as tooth extraction (TE).
OBJECTIVE: This
study aimed to evaluate the effect of selenium (SEL) administration on the prevention of osteonecrosis of the jaw in an MRONJ animal model.
UNASSIGNED: This
study was a longitudinal in vivo animal
study using a TE model in a sample of 48 Wistar rats.
METHODS: The predictor variables were SEL exposure, timing of SEL exposure, and zoledronic acid (ZOL) exposure. The animals were randomly assigned to 4 treatment groups (n = 12 per group): 1) saline (negative control), 2) ZOL (positive control), 3) SELpreop + ZOL, and 4) ZOL + SELpostop. The animals were administered saline (negative control) or ZOL (0.06 mg/kg, intraperitoneally) once a week for 5 weeks. All rats underwent TE at the end of the fifth week. SEL (0.3 mg/kg, intraperitoneally) was administered once daily for 15 days to the SELpreop + ZOL group before TE and to the ZOL + SELpostop group after TE. All animals were sacrificed at the end of the ninth week.
METHODS: The primary outcome variables were new bone area, necrotic bone area, fibrosis, new connective tissue formation, and inflammatory cell infiltration in the histopathological analysis, as well as angiogenesis and percentage of osteoblasts in the immunohistochemical analysis.
UNASSIGNED: There was none.
METHODS: Statistical analysis was conducted using the Kruskal-Wallis test, followed by post hoc Bonferroni-corrected Mann-Whitney U tests, with a significance level of P ≤ .05.
RESULTS: The new bone area was higher in the ZOL + SELpostop group (3.00 score) than in the saline group (0.58 ± 1.08 score, P < .001) and the ZOL group (0.82 ± 1.40 score, P = .001), while the necrotic bone area was lower in the ZOL + SELpostop group (0.08 ± 0.29 score) than in the ZOL group (2.82 ± 0.40 score, P < .001) and the SELpreop + ZOL group (1.67 ± 0.89 score, P = .007). The percentage of osteoblasts was higher in the ZOL + SELpostop group (18.73%) than in the saline group (8.63%, P < .001) and the ZOL group (0.07%, P < .001), and it was also higher in the SELpreop + ZOL group (18.49%) than in the ZOL group (0.07%, P < .001).
CONCLUSIONS: In conclusion SEL prevents MRONJ, with postoperative SEL demonstrating greater prevention effects. Given these findings, we hypothesize that SEL exposure may decrease the risk of MRONJ.