METHODS: Thirty-six Wistar rats were divided into three groups. Two experimental groups, Z and ZM, took zoledronic acid for 6 weeks prior to extraction of the left maxillary first molar. The Group ZM also was given methenolone enanthate continuously for 1 week before and 4 weeks after the extraction. The control group was not given any medication. The rats were euthanized 5 weeks after extraction. The extraction socket was evaluated clinically for bone exposure and histologically for inflammation, hyperemia, collagen fibers, epithelialization, number of osteoclasts, and empty lacunae.
RESULTS: Six rats died during the experimental research. The bone exposure rate, mean numbers of attached osteoclasts (in 40× magnification), and empty lacunae (in 100× magnification) were 0%, 4%, and 0.8% in Group C; 75%, 1%, and 8% in Group Z; and 10%, 2.1%, and 3% in Group ZM, respectively. Significant differences exist between all groups regarding the number of empty lacunae. There were significant differences between Group C/ZM and Group Z in terms of bone exposure rate, inflammation, hyperemia, collagen fiber organization, and epithelialization.
CONCLUSIONS: In our tested preclinical model, methenolone enanthate has shown potential for preventing medication-related osteonecrosis of the jaw.
方法:36只Wistar大鼠分为3组。两个实验组,Z和ZM,在拔除左上颌第一磨牙前服用唑来膦酸6周。ZM组还在提取前1周和提取后4周连续给予甲烯醇酮庚酸酯。对照组不给予任何药物治疗。在提取后5周将大鼠安乐死。对拔牙槽进行了骨暴露的临床评估和炎症的组织学评估,充血,胶原纤维,上皮化,破骨细胞的数量,和空的空洞。
结果:6只大鼠在实验研究中死亡。骨骼暴露率,附着破骨细胞的平均数量(放大40倍),空腔(放大100倍)为0%,4%,C组为0.8%;75%,1%,Z组为8%;10%,2.1%,ZM组为3%,分别。在空腔数方面,所有组之间存在显着差异。C/ZM组与Z组的骨暴露率差异有统计学意义,炎症,充血,胶原纤维组织,和上皮化。
结论:在我们测试的临床前模型中,甲烯醇酮庚酸酯已显示出预防与药物相关的颌骨坏死的潜力。