Due to ongoing changes and a decline in biodiversity, science today should rely on long-term species-based ecological studies. We have conducted a long-term ecological dynamics study on the water beetle family Scirtidae, which, although it is very abundant in benthic communities, is still poorly studied. The main objective of this study was to investigate the population aspects (composition, diversity, sex ratio) and ecological aspects (emergence patterns, seasonal dynamics and preferences for environmental factors) of the family Scirtidae over 15 years in Plitvice Lakes NP, Croatia. The study was conducted at three sites and in five different substrate types. A total of three taxa with different distributions were recorded in the study area: Hydrocyphon novaki and H. deflexicollis on the tufa barriers and the Elodes sp. in the spring area. The sex ratio was in favour of males in spring and early summer, while it shifted in favour of females towards the end of autumn. The abundance and emergence of the family were primarily determined by the environmental parameters which showed the greatest fluctuations over a period of 15 years: water temperature, water discharge and oxygen saturation. Our results clearly show that Scirtidae can be used as indicators of stream zonation and habitat quality. Based on the methodology and the results of this study, we conclude that Scirtidae should be used in future monitoring and protection measures in karst freshwater habitats in southeastern Europe.

BACKGROUND: The ongoing evolution of the Next Generation Sequencing (NGS) technologies has led to the production of genomic data on a massive scale. While tools for genomic data integration and analysis are becoming increasingly available, the conceptual and analytical complexities still represent a great challenge in many biological contexts.
RESULTS: To address this issue, we describe a six-steps tutorial for the best practices in genomic data integration, consisting of (1) designing a data matrix; (2) formulating a specific biological question toward data description, selection and prediction; (3) selecting a tool adapted to the targeted questions; (4) preprocessing of the data; (5) conducting preliminary analysis, and finally (6) executing genomic data integration.
CONCLUSIONS: The tutorial has been tested and demonstrated on publicly available genomic data generated from poplar (Populus L.), a woody plant model. We also developed a new graphical output for the unsupervised multi-block analysis, cimDiablo_v2, available at https://forgemia.inra.fr/umr-gdec/omics-integration-on-poplar , and allowing the selection of master drivers in genomic data variation and interplay.

Although uncommon, Epstein-Barr virus-related neurological disorders represent the seventh most frequent cause of infectious encephalitis in adults. The limited number of publications on EBV encephalitis mainly document isolated clinical cases. This study aimed to summarize published data on EBV encephalitis. A systematic literature search identified 97 EBV encephalitis cases. In the selected cases, EBV-related neurological disorders manifested as lymphocytic pleocytosis in the cerebrospinal fluid (CSF) with moderate hyperproteinorachia. The EBV PCR test was positive in 87% of the CSF samples, with wide-ranging viral loads. When encephalitis occurred in the context of past EBV infections, all of the EBV PCR tests on CSF samples were positive. On the contrary, negative EBV PCR tests on CSF samples occurred only in the context of primary infections. EBV PCR was rarely carried out on blood samples, contributing minimally to the diagnosis. For the treatment of EBV encephalitis, Aciclovir was used alone in 29% of cases, and in association with other drugs in 40% of cases. Ganciclovir (30%), corticoids (52%), and immunoglobulins (15%) were mainly used in association with other drugs. Cerebral imaging was abnormal in 69% of cases, mostly in the cerebellum and basal ganglia. This work highlights that the EBV PCR test on CSF samples is currently the main laboratory diagnostic test to diagnose EBV encephalitis. This diagnostic test is useful; however, it is imperfect. New complementary diagnostic tools, approved treatments, and standardized practices could improve patient management.

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Production, extraction, purification, and stabilization of integral membrane proteins are key steps for successful structural biology studies, in particular for X-ray crystallography or single particle microscopy. Here, we present the purification protocol of CntI from Pseudomonas aeruginosa, a new metallophore exporter of the Drug Metabolite Transporter (DMT) family involved in pseudopaline secretion. Subsequent to CntI purification, we optimized the buffer pH, salts, and additives by differential scanning fluorimetry (DSF), also known as Thermofluor Assay (TFA) or fluorescent thermal stability assay (FTSA), with the use of dye 1-AnilinoNaphthalene-8-Sulfonic acid (ANS), a fluorescent molecule compatible with detergents. After the buffer optimization, the purified CntI was analyzed by Size Exclusion Chromatography coupled with Multi-Angle Laser Light Scattering (SEC-MALLS), UV absorbance, and Refractive Index detectors, in order to determine the absolute molar mass of the protein-detergent complex, the detergent amount bound to the protein and the amount of protein-free detergent micelles. Altogether, these biophysical techniques give preliminary and mandatory information about the suitability of the purified membrane protein for further biophysical or structural investigations.

In our increasingly diverse society, it is important to examine the language used in scientific fields such as biology and immunology. Gender-neutral language aims to avoid gender-based assumptions and exclusionary language, promoting inclusivity and diversity, which are essential values in scientific research.

Improving the rate at which individuals enter into STEM careers remains a national concern. STEM fields are currently facing a crisis with respect to filling jobs with qualified workers, suggesting that STEM jobs are available yet remain open and waiting for qualified graduates. Although researchers have previously investigated variables such as demographics and attrition rates for the lack of STEM graduates available to fill these job vacancies, there is a critical need for additional research examining the impact of additional career-related variables. To explore the impact of a biology-focused career development course (CDC), we surveyed 277 biology majors in their final semester who participated in the CDC. Respondents were asked to describe their perceptions of the professional development modules contained within the CDC and to describe what they would have done differently had the CDC been available earlier in their academic career. We grounded data analysis in science and biology identity frameworks. In agreement with earlier identity studies, we found that engagement with the CDC enhanced student\'s performance/competence in biology and recognition as a biologist, two factors that are important for identity formation. Additionally, we show that students prefer to have the CDC earlier in their academic careers. Collectively, our data advance our understanding of career development of biology majors in two novel ways. First, we provide much needed qualitative data highlighting the mechanisms underlying the biology-focused CDC. Second, we provide both quantitative and qualitative data focused on the timing of the CDC, a topic which has not yet been purposely explored in biology.

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In recent years, driven by the support of national policies and societal needs for employments, talents in biology majors have been growing rapidly. To foster high-calibre biology talents for the society in the context of the \"double world-class initiative\" in higher education, this study analyzed the opinion of biology undergraduates in Huzhou University on employment and their professional recognition of biology majors. The aim of this study was to propose a high-quality employments-driven talent training mode for undergraduates in biology majors, so as to serve as a reference for the reform in training modes of other relevant majors.

We report the clinical case of a patient, 36 years old, presenting a major depressive disorder for more than 6 months. Somatic complaints (diarrhea and eczema) are also present. Thanks to a biological assessment, the diagnosis of celiac disease will be made and a strict gluten-free diet will be prescribed to the patient. This will allow the improvement of depressive symptoms and somatic complaints. This clinical case underlines the importance of the search for somatic causes in the development of a depressive symptomatology.
Nous rapportons le cas clinique d’une patiente, âgée de 36 ans, présentant un trouble dépressif majeur depuis plus de 6 mois. Des plaintes somatiques (diarrhée et eczéma) sont également présentes. Grâce à un bilan biologique, le diagnostic de maladie cœliaque sera posé et un régime strict sans gluten sera prescrit à la patiente. Celui-ci permettra l’amélioration de la symptomatologie dépressive et des plaintes somatiques. Ce cas clinique illustre l’importance de la recherche de causes somatiques dans la mise au point d’une symptomatologie dépressive.