工业和人类活动对重金属离子(HMIs)的环境污染有很大贡献,对水生生物有有害影响,植物,和动物,造成重大毒理学问题.市售的4,4'-二氨基-2,2'-二苯乙烯二磺酸(DSD)在重金属离子的检测中起着至关重要的作用,并且在现代科学的许多领域中都显着抑制了多种癌细胞。目前的研究旨在确保从环境中检测重金属离子和DSD的荧光成像治疗癌细胞。进行了荧光和紫外可见光谱分析,以感知探针DSD与几种重金属离子的选择行为,包括Fe2+,K1+,Co2+,Ni2+,Zn2+,Cd2+,Pb2+,Mn2+,Sn2+,Cr3+。此外,DSD接受了酶抑制的检查,如抗阿尔茨海默症,抗炎,抗氧化剂,抗癌,以及寻找多方面药物的抗菌活动。在乙酰胆碱酯酶(AChE)的体外酶抑制试验中,测试化合物已显示出剂量依赖性反应。丁酰胆碱酯酶(BChE),环氧合酶(COX),和脂氧合酶(LOX),以及抗氧化剂[DPPH=2,2-二苯基-1-吡啶酰肼和ABTS=2,2'-氮杂-双(3-乙基苯并噻唑啉-6-磺酸]。DSD在抑制乙酰胆碱酯酶(AChE)和丁酰胆碱酯酶(BChE)方面比常规药物加兰他敏更有效,IC50值为12.18和20.87μM,相当于标准药物。获得的结果表明,由于抑制了光诱导的电子转移途径(PET),DSD有可能成为检测Sn2离子而不是竞争金属离子的有效传感器。MTT(3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化铵)试验,证明DSD对脑癌细胞系NIH/3T3,HeLa和MCF-7具有很强的抗癌作用,IC50值分别为32.59、15.31和96.46μM。抗菌测试表明,DSD优于标准药物头孢克肟对白色念珠菌和铜绿假单胞菌,分别。这项研究为正在进行的有效治疗乳腺癌的研究做出了重大贡献。
Industrial and human activities contribute significantly to the environmental contamination of heavy metal ions (HMIs), which have detrimental effects on aquatic life, plants, and animals, causing major toxicological problems. The commercially available 4,4\'-diamino-2,2\'-stilbenedisulfonic acid (DSD) has been playing a vital role in the detection of heavy metal ions and has significantly inhibited a variety of cancer cells in numerous field of modern science. The current investigation aimed to ensure the detection of heavy metals ions from the environment and fluorescence imaging of DSD in the treatment of cancer cells. Fluorescence and UV-Visible spectroscopic analysis was performed to sense the selective behavior of the probe DSD with several heavy metal ions, including Fe2+, K1+, Co2+, Ni2+, Zn2+, Cd2+, Pb2+, Mn2+, Sn2+, and Cr3+. Furthermore, DSD was subjected to examine enzyme inhibition such as anti-Alzheimer, anti-inflammatory, antioxidant, anticancer, and antimicrobial activities in search of multifaceted drugs. Test compounds have demonstrated dose-dependent responses in the in-vitro enzyme inhibition assays for acetylcholinesterase (AChE), butyrylcholinesterase (
BChE), cyclooxygenase (COX), and lipoxygenase (LOX), as well as antioxidant [DPPH = 2,2-diphenyl-1-picrylhydrazyl and ABTS = 2,2\'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid]. The DSD were shown to be more effective than the conventional medication galantamine in inhibiting acetylcholinesterase (AChE) and butyrylcholinesterase (
BChE), with an IC50 value of 12.18 and 20.87 μM, which is equivalent to the standard drug. The results obtained has revealed that DSD has the potential to become an effective sensor for the detection of Sn2+ ions over competing metal ions due to the inhibition of photo-induced electron transfer pathway (PET). The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide tetrazolium) test, demonstrated that DSD had strong anticancer effects against the brain cancer cell line NIH/3T3, HeLa and MCF-7 with an IC50 value of 32.59, 15.31 and 96.46 μM respectively. The antimicrobial testing has shown that DSD outperforms the standard drug cefixime against Candida albicans and Pseudomonas aeruginosa, respectively. This study makes a substantial contribution to the ongoing search for efficient treatments for breast cancer.