背景:近年来,甲状腺癌越来越普遍。甲状腺的主要诊断方法是B超扫描,血清检测和穿刺检测。然而,这些方法是侵入性的和复杂的。迫切需要开发用于甲状腺癌诊断的非侵入性或微创方法。荧光法作为一种非侵入性检测方法备受关注。丁酰胆碱酯酶(BChE)是人体内常见的酶,和许多疾病影响其减少。我们发现BChE也是甲状腺癌的标志物。因此,通过定制的荧光探针探索BChE在甲状腺癌细胞中的表达,为研究甲状腺癌标志物表达以反映甲状腺状态提供有价值的实验数据和线索,具有一定的临床价值。
结果:在这项研究中,我们定制了一个名为Kang-BChE的荧光探针,这是很容易以高产率合成。实验结果表明,探针Kang-BChE可以在0-900UL-1的线性范围内检测BChE(R2=0.9963),检出限低至3.93UL-1(λex/em=550/689nm)。此外,Kang-BChE探针具有低细胞毒性,良好的特异性,并能完全消除乙酰胆碱酯酶(AChE)的干扰。Kang-BChE在血清回收率实验中检测复杂生物样品时表现出优异的稳定性(95.64-103.12%)。本研究是首次使用Kang-BChE研究BChE在甲状腺癌细胞(Tpc-1细胞)中的低表达。此外,我们观察到Tpc-1细胞中H2O2浓度与BChE活性呈正相关。
结论:Kang-BChE由于其出色的性能,有望成为监测复杂生物环境中BChE含量变化的重要工具。Kang-BChE具有优异的抗干扰能力,还可用于探索更多生物体中分子对BChE含量变化的影响。我们期望Kang-BChE能够在甲状腺癌的临床诊断和治疗中发挥重要作用。
BACKGROUND: Thyroid cancer has been increasingly prevalent in recent years. The main diagnostic methods for thyroid are B-ultrasound scan, serum detection and puncture detection. However, these methods are invasive and complex. It is a pressing need to develop non-invasive or minimally invasive methods for thyroid cancer diagnosis. Fluorescence method as a non-invasive detection method has attracted much attention. Butyrylcholinesterase (BChE) is a common enzyme in the human body, and many diseases affect its reduction. We found that
BChE is also a marker for thyroid cancer. Therefore, it is of certain clinical value to explore the expression of BChE in thyroid cancer cells through a customized fluorescent probe to provide valuable experimental data and clues for studying the expression of thyroid cancer marker to reflect thyroid status.
RESULTS: In this study, we customized a fluorescent probe named Kang-BChE, which is easy to synthesize with a high yield. The experimental results show that the probe Kang-
BChE can detect
BChE in the linear range of 0-900 U L-1 (R2 = 0.9963), and the detection limit is as low as 3.93 U L-1 (λex/em = 550/689 nm). In addition, Kang-BChE probes have low cytotoxicity, good specificity, and can completely eliminate interference from acetylcholinesterase (AChE). Kang-BChE showed excellent stability in the detection of complex biological samples in serum recovery experiments (95.64-103.12 %). This study was the first time using Kang-
BChE to study the low expression of
BChE in thyroid cancer cells (Tpc-1 cells). In addition, we observed that H2O2 concentration in Tpc-1 cells was positively correlated with BChE activity.
CONCLUSIONS: Kang-
BChE is expected to be an important tool for monitoring the change of
BChE content in complex biological environments due to its excellent performance. Kang-
BChE can also be used to explore the influence of molecules in more organisms on the change of
BChE content due to its excellent anti-interference ability. We expect that Kang-BChE can play a significant role in the clinical diagnosis and treatment of thyroid cancer.