Abstract：
The sensitivity of phosphorylation site identification by mass spectrometry (MS)-based phosphoproteomics has improved significantly. However, the lack of kinase-substrate relationship (KSR) data has hindered improvement of the range and accuracy of kinase activity prediction using phosphoproteome data. We herein describe the application of a systematic identification of KSR by integrated phosphoproteome and interactome analysis using doxycycline (Dox)-induced target kinase-overexpressing HEK-293 cells.
摘要：
通过基于质谱（MS）的磷酸化蛋白质组学进行磷酸化位点鉴定的灵敏度显着提高。然而,缺乏激酶-底物关系(KSR)数据阻碍了使用磷酸蛋白质组数据预测激酶活性的范围和准确性的提高.我们在此描述了使用多西环素（Dox）诱导的靶激酶过表达HEK-293细胞通过整合的磷酸蛋白质组和相互作用组分析对KSR进行系统鉴定的应用。
