{Reference Type}: Journal Article
{Title}: Systematic Identification of Kinase-Substrate Relationship by Integrated Phosphoproteome and Interactome Analysis.
{Author}: Muraoka S;Adachi J;
{Journal}: Methods Mol Biol
{Volume}: 2823
{Issue}: 0
{Year}: 2024
暂无{DOI}: 10.1007/978-1-0716-3922-1_2
{Abstract}: The sensitivity of phosphorylation site identification by mass spectrometry (MS)-based phosphoproteomics has improved significantly. However, the lack of kinase-substrate relationship (KSR) data has hindered improvement of the range and accuracy of kinase activity prediction using phosphoproteome data. We herein describe the application of a systematic identification of KSR by integrated phosphoproteome and interactome analysis using doxycycline (Dox)-induced target kinase-overexpressing HEK-293 cells.