%0 Journal Article
%T Systematic Identification of Kinase-Substrate Relationship by Integrated Phosphoproteome and Interactome Analysis.
%A Muraoka S
%A Adachi J
%J Methods Mol Biol
%V 2823
%N 0
%D 2024
%M 39052211
暂无%R 10.1007/978-1-0716-3922-1_2
%X The sensitivity of phosphorylation site identification by mass spectrometry (MS)-based phosphoproteomics has improved significantly. However, the lack of kinase-substrate relationship (KSR) data has hindered improvement of the range and accuracy of kinase activity prediction using phosphoproteome data. We herein describe the application of a systematic identification of KSR by integrated phosphoproteome and interactome analysis using doxycycline (Dox)-induced target kinase-overexpressing HEK-293 cells.