Abstract：
Zn2+ plays a vital role in regulating various life processes, such as gene expression, cell signaling, and brain function. In this study, a near-infrared fluorescent probe AXS was synthesized to detect Zn2+ with good fluorescence specificity, high selectivity, and high sensitivity; the detection limit of Zn2+ was 6.924 × 10-11 M. The mechanism of Zn2+ recognition by the AXS probe was investigated by 1H nuclear magnetic resonance titrations, UV-visible spectroscopy, fluorescence spectroscopy, Fourier-transform infrared spectroscopy, and high-resolution mass spectrometry. Test paper experiments showed that the AXS probe could detect Zn2+ in real samples. In addition, quantitative and qualitative detection of Zn2+ in common foodstuffs was achieved. For portable Zn2+ detection, a smartphone detection platform was also developed based on the AXS probe. Importantly, the AXS probe showed good bioimaging capabilities in Caenorhabditis elegans and mice.
摘要：
Zn2+在调节各种生命过程中起着至关重要的作用,如基因表达，细胞信号，和大脑功能。在这项研究中,合成了具有良好荧光特异性的近红外荧光探针AXS,高选择性,灵敏度高；Zn2+的检出限为6.924×10-11M。通过1H核磁共振滴定研究了AXS探针对Zn2+的识别机理，紫外可见光谱，荧光光谱法，傅里叶变换红外光谱,和高分辨率质谱。试纸实验表明，AXS探针可以检测实际样品中的Zn2+。此外,实现了对常见食品中Zn2+的定量和定性检测。对于便携式Zn2+检测，还开发了基于AXS探针的智能手机检测平台.重要的是,AXS探针在秀丽隐杆线虫和小鼠中显示出良好的生物成像能力。
