Abstract：
Global warming may reshape seasonal changes in microbial community diversity and co-occurrence network patterns, with significant implications for terrestrial ecosystem function. We conducted a 2-year in situ field simulation of the effects of warming on the seasonal dynamics of soil microbial communities in a northern subtropical Quercus acutissima forest. Our study revealed that warming had no significant effect on the richness or diversity of soil bacteria or fungi in the growing season, whereas different warming gradients had different effects on their diversity in the nongrowing season. Warming also changed the microbial community structure, increasing the abundance of some thermophilic microbial species and decreasing the abundance of some symbiotrophic microorganisms. The co-occurrence network analysis of the microbial community showed that warming decreased the complexity of the intradomain network in the soil bacterial community in the growing and nongrowing seasons but increased it in the fungal community. Moreover, increasing warming temperatures increased the complexity of the interdomain network between bacteria and fungi in the growing season but decreased it in the nongrowing season, and the keystone species in the interdomain network changed with warming. Warming also reduced the proportion of positive microbial community interactions, indicating that warming reduced the mutualism, commensalism, and neutralism of microorganisms as they adapted to soil environmental stress. The factors affecting the fungal community varied considerably across warming gradients, with the bacterial community being significantly affected by soil temperature, MBC, NO3--N and NH4+-N, moreover, SOC and TN significantly affected fungal communities in the 4 °C warming treatment. These results suggest that warming increases seasonal differences in the diversity and complexity of soil microbial communities in the northern subtropical region, significantly influencing soil dynamic processes regulating forest ecosystems under global warming.
摘要：
全球变暖可能会重塑微生物群落多样性和共生网络模式的季节性变化，对陆地生态系统功能具有重要意义。我们进行了为期2年的原位现场模拟，研究了变暖对北部亚热带麻棘树林土壤微生物群落季节性动态的影响。我们的研究表明，在生长季节，气候变暖对土壤细菌或真菌的丰富度或多样性没有显著影响。而在非生长季节，不同的增温梯度对其多样性有不同的影响。变暖也改变了微生物群落结构，增加一些嗜热微生物物种的丰度，减少一些共生微生物的丰度。微生物群落的共现网络分析表明，在生长和非生长季节，变暖降低了土壤细菌群落中生物内网络的复杂性，但在真菌群落中却增加了复杂性。此外，在生长季节，温度升高增加了细菌和真菌之间域间网络的复杂性，但在非生长季节降低了复杂性，域间网络中的重点物种随着变暖而变化。变暖也降低了积极的微生物群落相互作用的比例，表明变暖减少了共生关系，共产主义,以及微生物适应土壤环境胁迫时的中立性。影响真菌群落的因素在变暖梯度上有很大差异，细菌群落受到土壤温度的显著影响，MBC,NO3--N和NH4+-N,此外,在4°C加温处理中，SOC和TN显着影响真菌群落。.这些结果表明，变暖增加了北亚热带地区土壤微生物群落多样性和复杂性的季节性差异，全球变暖对调节森林生态系统的土壤动态过程有显著影响。
