由于其耐用的木材而利用了蕨类植物,为野生动物提供食物,保护生物多样性,贡献生态,药用,以及对生态系统和景观的美学益处。在这项研究中,我们的目标是使用三种蕨类植物的树皮(Q.dalechampi,问:fraineto,和Q.petraea)用于合成银和金纳米颗粒(AgNP和AuNP)。橡树皮的水提物。既充当还原剂又充当稳定剂,促进AuNP的快速合成(AuQD,AuQF,和AuQP)和AgNPs(AgQD,AgQF,和AgQP)。使用UV-vis光谱对获得的纳米颗粒进行表征,TEM,DLS,和FTIR。表征显示纳米粒子表现出各种形状,如多边形,三角形,和球形,AuNP的尺寸范围为14至24nm,AgNP的尺寸范围为45-70nm。总酚含量通过光谱法评估,同时使用UPLC-PDA鉴定和定量了几种单独的酚类化合物。此外,我们评估了抗氧化剂,抗菌,以及AuNPs的抗真菌能力,AgNPs,和原始提取物。对于原始提取物,观察到最高的抗氧化活性,其次是AgNPs和AuNPs,而在AgQP中观察到最有效的抗菌和抗真菌活性。此外,在人角质形成细胞系(HaCaT)中检查细胞毒性。结果表明AuNP没有细胞毒性作用,而AgNP和原始提取物在孵育48小时后表现出细胞毒性作用。这项研究强调了Quercus树皮提取物在金属纳米颗粒的绿色合成及其随后的生物活性评估中的多方面用途。为它们在各个领域的应用提出了有希望的观点,同时敦促谨慎考虑它们的细胞毒性影响。
Quercus species are utilized for their durable wood, providing sustenance for wildlife, conserving biodiversity, and contributing ecological, medicinal, and esthetic benefits to ecosystems and landscapes. In this study, we aimed to use the bark of three
Quercus species (Q. dalechampi, Q. fraineto, and Q. petraea) for the synthesis of silver and gold nanoparticles (AgNPs and AuNPs). The aqueous extracts from the bark of
Quercus sp. acted both as reducing and stabilizing agent, facilitating the rapid synthesis of AuNPs (AuQD, AuQF, and AuQP) and AgNPs (AgQD, AgQF, and AgQP). The obtained nanoparticles were characterized using UV-vis spectroscopy, TEM, DLS, and FTIR. Characterizations revealed that the nanoparticles exhibited a variety of shapes, such as polygonal, triangular, and spherical forms, with sizes ranging between 14 and 24 nm for AuNPs and 45-70 nm for AgNPs. The total phenolic content was assessed through spectroscopic methods, while several individual phenolic compounds were identified and quantified using UPLC-PDA. Furthermore, we assessed the antioxidant, antibacterial, and antifungal capacities of AuNPs, AgNPs, and raw extracts. The highest antioxidant activity was observed for raw extracts, followed by AgNPs and AuNPs, while the most potent antibacterial and antifungal activity was observed in AgQP. Moreover, cytotoxicity was examined in a human keratinocyte cell line (HaCaT). The results indicated no cytotoxic effects for AuNPs, while AgNPs and the raw extracts exhibited cytotoxic effects after 48 h of incubation. This research underscores the multifaceted utility of
Quercus bark extracts in the green synthesis of metallic nanoparticles and their subsequent bioactivity assessment, suggesting promising perspectives for their application in various fields while urging cautious consideration of their cytotoxic implications.