PDF(Pubmed)
Abstract：
Leaf senescence is essential for the growth and development of deciduous trees in the next season. Larix gmelinii, a deciduous coniferous tree, exhibits its most distinctive feature by turning yellow in the autumn and eventually shedding its leaves, resulting in significant changes in its appearance during the fall. Lysine acetylation plays an important role in diverse cellular processes; however, limited knowledge is available regarding acetylations in the needle senescence of L. gmelinii. In this study, the proteomics and acetylated modification omics of two phenotypic leaves, yellow and green (senescent and non-senescent) needles, were analyzed before autumn defoliation. In total, 5022 proteins and 4469 unique acetylation sites in 2414 lysine acylated proteins were identified, and this resulted in the discovery of 1335 differentially expressed proteins (DEPs) and 605 differentially expressed acetylated proteins (DAPs) in yellow versus green needles. There are significant differences between the proteome and acetylome; only 269 proteins were found to be DEP and DAP, of which 136 proteins were consistently expressed in both the DEP and DAP, 91 proteins were upregulated, and 45 proteins were down-regulated. The DEPs participate in the metabolism of starch and sucrose, while the DAPs are involved in glycolysis and the tricarboxylic acid cycle. Among them, DEPs underwent significant changes in glycolysis and citric acid cycling. Most of the enzymes involved in glycolysis and the citrate cycle were acetylated. DAPs were down-regulated in glycolysis and up-regulated in the citrate cycle. In all, the results of this study reveal the important role of lysine acetylation in the senescence of L. gmelinii needles and provide a new perspective for understanding the molecular mechanism of leaf senescence and tree seasonal growth.
摘要：
叶片衰老对于下一季落叶乔木的生长发育至关重要。兴安落叶松,一棵落叶针叶树，通过在秋天变黄并最终脱落叶子来展示其最鲜明的特征，导致其外观在秋季的显著变化。赖氨酸乙酰化在不同的细胞过程中起着重要的作用;然而，关于格氏乳杆菌针叶衰老中乙酰化的知识有限。在这项研究中,两种表型叶片的蛋白质组学和乙酰化修饰组学，黄色和绿色（衰老和非衰老）针，在秋季落叶之前进行了分析。总的来说,在2414个赖氨酸酰化蛋白中鉴定出5022个蛋白和4469个独特的乙酰化位点，这导致在黄色与绿色针中发现了1335种差异表达的蛋白质（DEP）和605种差异表达的乙酰化蛋白质（DAP）。蛋白质组和乙酰组之间存在显著差异;只有269个蛋白质被发现是DEP和DAP,其中136种蛋白质在DEP和DAP中一致表达，91种蛋白质上调，45种蛋白质被下调。DEPs参与淀粉和蔗糖的代谢,而DAP参与糖酵解和三羧酸循环。其中,DEP在糖酵解和柠檬酸循环中发生了显着变化。参与糖酵解和柠檬酸盐循环的大多数酶是乙酰化的。DAP在糖酵解中下调，在柠檬酸盐循环中上调。总之,本研究结果揭示了赖氨酸乙酰化在叶衰老中的重要作用，为理解叶衰老和树木季节生长的分子机制提供了新的视角。
