作为森林生态系统的地上和地下组成部分之间的重要联系,土壤细菌群落对植物群落和土壤条件的变化极为敏感。为了研究建设性物种差异对针叶林土壤细菌群落的影响,我们在大兴安岭落叶松林的国际监测区进行了植被调查,并根据这项调查计算了落叶松的重要值,以确定实验组。随后,我们收集土壤样本进行高通量测序,分析土壤细菌群落组成和多样性的变化,哪些因素影响了他们。结果表明,落叶松重要值不同的针叶林具有异质性生境,其中土壤AP含量显著增加,和SOC,MBC,pH值,C/N含量显著降低(p<0.05)。总共32门,91班,200个订单,308个家庭,496属,通过测序获得了975种土壤细菌。其中,变形杆菌,放线菌,酸杆菌是主要的门,分枝杆菌是优势属,每个细菌组的相对丰度是不同的。土壤细菌β多样性差异极显著(p=0.001),SOC,C/N,MBC,AP,TN,pH值,AN,WC是主要影响因素。功能预测分析表明,化学异型和需氧化学异型是主要的细菌功能群,各功能组的相对丰度差异有统计学意义(p<0.05)。总的来说,建设性物种差异的针叶林具有异质生境,改变了社区组成,β多样性,和土壤细菌的潜在功能。
As a crucial link between the aboveground and belowground components of forest ecosystems, soil bacterial communities are extremely sensitive to changes in plant communities and soil conditions. To investigate the impact of the difference of constructive species on soil bacterial communities in taiga forests, we conducted a vegetation survey at the international monitoring plot of the Larix gmelinii forests in the Great Khingan Mountains and calculated the important value of Larix gmelinii to determine experimental groups based on this survey. Subsequently, we collected soil samples for high-throughput sequencing to analyze how the soil bacterial community composition and diversity changed, and which factors affected them. The results showed that taiga forests with different important values of Larix gmelinii had heterogeneous habitats, in which the soil AP content significantly increased, and the SOC, MBC, pH, and C/N content decreased significantly (p < 0.05). A total of 32 phyla, 91 classes, 200 orders, 308 families, 496 genera, and 975 species of soil bacteria were obtained by sequencing. Among them, Proteobacteria, Actinobacteriota, and Acidobacteriota were the dominant phyla, and Mycobacterium was the dominant genus, and the relative abundance of each bacterial group was varied. The beta diversity of soil bacteria showed extremely significant differences (p = 0.001), with SOC, C/N, MBC, AP, TN, pH, AN, and WC being the main influencing factors. Functional prediction analysis showed that chemoheterotrophy and aerobic chemoheterotrophy were the main bacterial functional groups, and the relative abundance of each functional group was significantly different (p < 0.05). Overall, taiga forests with differences in constructive species had heterogeneous habitats, which changed the community composition, beta diversity, and potential functions of soil bacteria.