赖氨酸乙酰化修饰是一种动态且可逆的翻译后修饰,在病原菌的代谢和致病性中起着重要作用。溶藻弧菌是水产养殖中常见的致病菌,和胆盐可以触发细菌毒力的表达。然而,在胆汁盐胁迫下,赖氨酸乙酰化在溶藻弧菌中的作用鲜为人知。在这项研究中,通过乙酰赖氨酸抗体富集和高分辨率质谱,在胆汁盐胁迫下在溶藻弧菌中鉴定了689种蛋白质上的1,315个乙酰化肽。生物信息学分析发现,肽基序****A*Kac*****和*****Kac****A*高度保守,蛋白赖氨酸乙酰化参与调节各种细胞生物学过程,维持细菌的正常生命活动,比如核糖体,氨酰基-tRNA生物合成,脂肪酸代谢,双组分系统,和细菌分泌系统。Further,还发现22个乙酰化蛋白通过分泌系统与胆汁盐胁迫下溶藻弧菌的毒力有关,趋化性和运动性,和坚持。最后,比较未处理和处理的胆汁盐应激赖氨酸乙酰化蛋白,发现有240个重叠的蛋白质,发现氨基糖和核苷酸糖代谢,β-内酰胺抗性,脂肪酸降解,碳代谢,不同环境中的微生物代谢途径在胆盐胁迫下显著富集。总之,本研究是对胆盐胁迫下溶藻弧菌赖氨酸乙酰化的整体分析,特别是许多毒力因子也有乙酰化。
Lysine acetylation modification is a dynamic and reversible post-translational modification, which plays an important role in the metabolism and pathogenicity of pathogenic bacteria. Vibrio alginolyticus is a common pathogenic bacterium in aquaculture, and bile salt can trigger the expression of bacterial virulence. However, little is known about the function of lysine acetylation in V. alginolyticus under bile salt stress. In this study, 1,315 acetylated peptides on 689 proteins were identified in V. alginolyticus under bile salt stress by acetyl-lysine antibody enrichment and high-resolution mass spectrometry. Bioinformatics analysis found that the peptides motif ****A*Kac**** and *******Kac****A* were highly conserved, and protein lysine acetylation was involved in regulating various cellular biological processes and maintaining the normal life activities of bacteria, such as ribosome, aminoacyl-tRNA biosynthesis, fatty acid metabolism, two-component system, and bacterial secretion system. Further, 22 acetylated proteins were also found to be related to the virulence of V. alginolyticus under bile salt stress through secretion system, chemotaxis and motility, and adherence. Finally, comparing un-treated and treated with bile salt stress lysine acetylated proteins, it was found that there were 240 overlapping proteins, and found amino sugar and nucleotide sugar metabolism, beta-Lactam resistance, fatty acid degradation, carbon metabolism, and microbial metabolism in diverse environments pathways were significantly enriched in bile salt stress alone. In conclusion, this study is a holistic analysis of lysine acetylation in V. alginolyticus under bile salt stress, especially many virulence factors have also acetylated.