这里,我们提出了BtS2160-1的全基因组序列,这是一种潜在的灭蚊模型菌株的替代品,Bti.BtS2160-1中包含一个染色体基因组和四个大型质粒,并且鉴定出13个编码预测杀虫晶体蛋白的预测基因聚集在一个质粒pS2160-1p2上,该质粒包含两个设计为PAI-1的致病岛(PAIs)(Cry54Ba,Cry30Ea4,Cry69Aa-like,Cry50Ba2样,Cry4Ca1样,Cry30Ga2,Cry71Aa样,Cry72Aa-like,Cry70Aa-like,Cyt1Da2样和Vpb4C1样)和PAI-2(Cyt1Aa样,和Tpp80Aa1一样)。簇似乎代表类似于致病性岛的杀蚊毒素岛。使用LTQ-OrbitrapLC-MS/MS通过全蛋白质组分析证实了13个预测基因中的10个的转录/翻译。总之,本研究确定了苏云金芽孢杆菌中存在灭蚊毒素岛,并为了解苏云金芽孢杆菌的杀虫机理提供了重要的基因组信息。
Here, we present the whole genome sequence of Bt S2160-1, a potential alternative to the mosquitocidal model strain, Bti. One chromosome genome and four mega-plasmids were contained in Bt S2160-1, and 13 predicted genes encoding predicted insecticidal crystal proteins were identified clustered on one plasmid pS2160-1p2 containing two pathogenic islands (PAIs) designed as PAI-1 (Cry54Ba, Cry30Ea4, Cry69Aa-like, Cry50Ba2-like, Cry4Ca1-like, Cry30Ga2, Cry71Aa-like, Cry72Aa-like, Cry70Aa-like, Cyt1Da2-like and Vpb4C1-like) and PAI-2 (Cyt1Aa-like, and Tpp80Aa1-like). The clusters appear to represent mosquitocidal toxin islands similar to pathogenicity islands. Transcription/translation of 10 of the 13 predicted genes was confirmed by whole-
proteome analysis using LTQ-Orbitrap LC-MS/MS. In summary, the present study identified the existence of a mosquitocidal toxin island in Bacillus thuringiensis, and provides important genomic information for understanding the insecticidal mechanism of B. thuringiensis.