■锯缘石杉是一种传统的中草药,因其生产石杉碱甲(HupA)而备受关注。HupA已显示出治疗阿尔茨海默病(AD)的希望。然而,HupA的生物合成途径和分子机制尚不清楚。
■进行了整合转录组和代谢组分析,以揭示与锯缘石杉树中HupA生物合成和抗氧化活性相关的分子机制。
■HT(体外H.serratathallus)比WH(野生H.serrata)表现出更高的抗氧化活性和更低的细胞毒性。通过层次聚类分析和qRT-PCR验证,检测到7个与HupA生物合成相关的重要酶基因和13个转录因子(TFs)。其中,CYP(细胞色素P450)和CAO(铜胺氧化酶)的平均|log2FC|值最大。代谢组学分析鉴定了12种参与HupA生物合成的代谢物和29种与抗氧化活性相关的代谢物。KEGG共富集分析显示,托烷,哌啶和吡啶生物碱的生物合成参与了HupA的生物合成途径。此外,类苯丙素,苯丙氨酸,发现黄酮类生物合成途径可调节锯缘的抗氧化活性。该研究还确定了与抗氧化活性调节相关的七个重要基因,包括PrAO(伯胺氧化酶)。基于上述联合分析,构建了锯缘HupA的生物合成途径和潜在的抗氧化机制。
■通过差异转录组和代谢组分析,鉴定了参与HupA生物合成和抗氧化相关的DEGs和DAMs,构建了与锯缘石杉HupA生物合成和抗氧化相关的潜在代谢途径。这项研究将为HupA生物合成机制和锯缘H.serratathallus的药用价值提供有价值的见解。
UNASSIGNED: Huperzia serrata is a traditional Chinese herb that has gained much attention for its production of Huperzine A (HupA). HupA has shown promise on treating Alzheimer\'s disease (AD). However, the biosynthetic pathway and molecular mechanism of HupA in H. serrata are still not well understood.
UNASSIGNED: Integrated transcriptome and metabolome analysis was performed to reveal the molecular mechanisms related to HupA biosynthesis and antioxidant activity in Huperzia serrata.
UNASSIGNED: HT (in vitro H. serrata thallus) exhibits higher antioxidant activity and lower cytotoxicity than WH (wild H. serrata). Through hierarchical clustering analysis and qRT-PCR verification, 7 important enzyme genes and 13 transcription factors (TFs) related to HupA biosynthesis were detected. Among them, the average |log2FC| value of CYP (Cytochrome P450) and CAO (Copper amine oxidase) was the largest. Metabolomic analysis identified 12 metabolites involved in the HupA biosynthesis and 29 metabolites related to antioxidant activity. KEGG co-enrichment analysis revealed that tropane, piperidine and pyridine alkaloid biosynthesis were involved in the HupA biosynthesis pathway. Furthermore, the phenylpropanoid, phenylalanine, and flavonoid biosynthesis pathway were found to regulate the antioxidant activity of H. serrata. The study also identified seven important genes related to the regulation of antioxidant activity, including PrAO (primary-amine oxidase). Based on the above joint analysis, the biosynthetic pathway of HupA and potential mechanisms of antioxidant in H. serrata was constructed.
UNASSIGNED: Through differential transcriptome and metabolome analysis, DEGs and DAMs involved in HupA biosynthesis and antioxidant-related were identified, and the potential metabolic pathway related to HupA biosynthesis and antioxidant in Huperzia serrata were constructed. This study would provide valuable insights into the HupA biosynthesis mechanism and the H. serrata thallus medicinal value.