PDF(Pubmed)
Abstract：
BACKGROUND: Canine circovirus (CanineCV) is a single-stranded circular DNA virus that infects domestic and wild canids in many countries. CanineCV is associated with gastroenteritis and diarrhea, respiratory disease, and generalized vasculitis leading to a fatal event. The Capsid protein (Cap) is a structural protein of the virus which has high genetic variability and plays a role in the canine immune response. In this study, we cloned the full-length CanineCV Capsid gene (Cap). In-silico analyses were used to explore the genomic and amino acid variability and natural selection acting on the Cap gene. The immune relevance for T-cell and B-cell epitopes was predicted by the immunoinformatic approach.
RESULTS: According to the Cap gene, our results showed that CanineCV was separated into five phylogenetic groups. The obtained CanineCV strain from this study was grouped with the previously discovered Thai strain (MG737385), as supported by a haplotype network. Entropy analyses revealed high nucleotide and amino acid variability of the Capsid region. Selection pressure analysis revealed four codons at positions 24, 50, 103, and 111 in the Cap protein evolved under diversifying selection. Prediction of B-cell epitopes exhibited four consensus sequences based on physiochemical properties, and eleven peptide sequences were predicted as T-cell epitopes. In addition, the positive selection sites were located within T-cell and B-cell epitopes, suggesting the role of the host immune system as a driving force in virus evolution.
CONCLUSIONS: Our study provides knowledge of CanineCV genetic diversity, virus evolution, and potential epitopes for host cell immune response.
RESULTS: According to the Cap gene, our results showed that CanineCV was separated into five phylogenetic groups. The obtained CanineCV strain from this study was grouped with the previously discovered Thai strain (MG737385), as supported by a haplotype network. Entropy analyses revealed high nucleotide and amino acid variability of the Capsid region. Selection pressure analysis revealed four codons at positions 24, 50, 103, and 111 in the Cap protein evolved under diversifying selection. Prediction of B-cell epitopes exhibited four consensus sequences based on physiochemical properties, and eleven peptide sequences were predicted as T-cell epitopes. In addition, the positive selection sites were located within T-cell and B-cell epitopes, suggesting the role of the host immune system as a driving force in virus evolution.
CONCLUSIONS: Our study provides knowledge of CanineCV genetic diversity, virus evolution, and potential epitopes for host cell immune response.
摘要：
背景：犬圆环病毒（CanineCV）是一种单链环状DNA病毒，在许多国家感染家犬和野生犬科动物。CanineCV与胃肠炎和腹泻有关，呼吸道疾病,和导致致命事件的全身性血管炎。衣壳蛋白（Cap）是病毒的结构蛋白，具有很高的遗传变异性，在犬的免疫反应中起作用。在这项研究中,我们克隆了全长CanineCV衣壳基因(Cap)。计算机分析用于探索基因组和氨基酸变异性以及作用于Cap基因的自然选择。通过免疫信息学方法预测T细胞和B细胞表位的免疫相关性。
结果：根据Cap基因，我们的结果表明，canineCV分为五个系统发育组。从这项研究中获得的CanineCV菌株与先前发现的泰国菌株（MG737385）分组，由单倍型网络支持。熵分析揭示了衣壳区的高核苷酸和氨基酸变异性。选择压力分析揭示了在多样化选择下进化的Cap蛋白中位置24、50、103和111处的四个密码子。B细胞表位的预测显示四个基于理化性质的共有序列，和11个肽序列被预测为T细胞表位。此外,阳性选择位点位于T细胞和B细胞表位内，表明宿主免疫系统在病毒进化中的作用。
结论：我们的研究提供了犬科动物遗传多样性的知识，病毒进化,和宿主细胞免疫应答的潜在表位。
结果：根据Cap基因，我们的结果表明，canineCV分为五个系统发育组。从这项研究中获得的CanineCV菌株与先前发现的泰国菌株（MG737385）分组，由单倍型网络支持。熵分析揭示了衣壳区的高核苷酸和氨基酸变异性。选择压力分析揭示了在多样化选择下进化的Cap蛋白中位置24、50、103和111处的四个密码子。B细胞表位的预测显示四个基于理化性质的共有序列，和11个肽序列被预测为T细胞表位。此外,阳性选择位点位于T细胞和B细胞表位内，表明宿主免疫系统在病毒进化中的作用。
结论：我们的研究提供了犬科动物遗传多样性的知识，病毒进化,和宿主细胞免疫应答的潜在表位。
