RESULTS: According to the Cap gene, our results showed that CanineCV was separated into five phylogenetic groups. The obtained CanineCV strain from this study was grouped with the previously discovered Thai strain (MG737385), as supported by a haplotype network. Entropy analyses revealed high nucleotide and amino acid variability of the Capsid region. Selection pressure analysis revealed four codons at positions 24, 50, 103, and 111 in the Cap protein evolved under diversifying selection. Prediction of B-cell epitopes exhibited four consensus sequences based on physiochemical properties, and eleven peptide sequences were predicted as T-cell epitopes. In addition, the positive selection sites were located within T-cell and B-cell epitopes, suggesting the role of the host immune system as a driving force in virus evolution.
CONCLUSIONS: Our study provides knowledge of CanineCV genetic diversity, virus evolution, and potential epitopes for host cell immune response.
结果:根据Cap基因,我们的结果表明,canineCV分为五个系统发育组。从这项研究中获得的CanineCV菌株与先前发现的泰国菌株(MG737385)分组,由单倍型网络支持。熵分析揭示了衣壳区的高核苷酸和氨基酸变异性。选择压力分析揭示了在多样化选择下进化的Cap蛋白中位置24、50、103和111处的四个密码子。B细胞表位的预测显示四个基于理化性质的共有序列,和11个肽序列被预测为T细胞表位。此外,阳性选择位点位于T细胞和B细胞表位内,表明宿主免疫系统在病毒进化中的作用。
结论:我们的研究提供了犬科动物遗传多样性的知识,病毒进化,和宿主细胞免疫应答的潜在表位。