本研究的目的是调查频率,遗传变异,和绵羊和山羊胎儿的小反刍动物病毒(PPRV)的系统发育。在2014年和2017年期间,土耳其共收集了1054个胚胎/胎儿。使用实时RT-PCR测定法检测PPRVRNA。通过对融合(F)蛋白和核蛋白(N)基因片段进行测序,对PPRV田间分离株进行了遗传表征和系统发育分析。还从已经流产并且发现其胚胎/胎儿为PPRV阳性的母羊(n=83)和保姆山羊(n=3)收集样品。还测试了PPRV阳性胚胎/胎儿是否存在单核细胞增生李斯特菌,弯曲杆菌属。,伯内蒂柯西拉,流产衣原体,布鲁氏菌属。,赤班病毒,爱诺病毒,蓝舌病毒,边境病病毒,牛病毒性腹泻病毒,缓存谷病毒,和Schmallenberg病毒.在1054个胚胎/胎儿的123个(11.7%)中检测到PPRVRNA,83只(94%)母羊的78只和3只(100%)保姆山羊。在7和12个PPRV阳性绵羊胎儿中检测到边界病病毒RNA和流产衣原体DNA,分别,而未检测到其他细菌和病毒试剂。系统发育,本研究中的田间分离株属于谱系IV,并与本研究中考虑的其他谱系IV菌株相比,它们在F和N基因序列中显示了1和5个新的氨基酸取代,分别。研究结果表明,PPRV在流产中起着重要作用。因此,在绵羊和山羊流产中需要考虑PPRV。
The aim of the present study was to investigate the frequency, genetic variability, and phylogeny of the peste des petits ruminants virus (PPRV) in ovine and caprine fetuses. During 2014 and 2017, a total of 1054 embryos/fetuses were collected in Turkey. A real-time RT-PCR assay was used for the detection of the PPRV RNA. Genetic characterization and phylogenetic analysis of the PPRV field isolates were conducted by sequencing fusion (F) protein and nucleoprotein (N) gene segments. Samples were also collected from ewes (n = 83) and nanny goats (n = 3) that had aborted and whose embryos/fetuses were found to be PPRV positive. PPRV positive embryos/fetuses were also tested for the presence of Listeria monocytogenes, Campylobacter spp., Coxiella burnetii, Chlamydophila abortus, Brucella spp., akabane virus, aino virus, bluetongue virus, border disease virus, bovine viral diarrhea virus, Cache Valley virus, and Schmallenberg virus. PPRV RNA was detected in 123 (11.7 %) of the 1054 embryos/fetuses, 78 of the 83 (94 %) ewes and 3 (100 %) nanny goats. Border disease virus RNA and Chlamydophila abortus DNA were detected in 7 and 12 PPRV positive sheep fetuses, respectively, while other bacterial and viral agents were not detected. Phylogenetically, the field isolates in this study belong to lineage IV, and compared to other strains of lineage IV considered in this study, they showed 1 and 5 new amino acid substitutions in the F and N gene sequences, respectively. The results of the study suggest that PPRV plays an important role in abortion. Therefore, PPRV needs to be taken into consideration in sheep and goats abortions.