Abstract：
Between March and October 2022, a peak of detection of Bordetella parapertussis by qPCR, real-time PCR was observed in France.Hypothesis/Gap Statement. Whether this peak was due to resurgence from previous circulating lineages or reintroduction into the country was unknown.Objective. The objective of this study is to understand B. parapertussis-transient increase observed in France in 2022 whereas it had virtually stopped being reported since the start of the COVID-19 pandemic in 2020.Methods. We analysed real-time PCR (qPCR) data from the two largest French outpatient laboratories performing whooping cough diagnosis and characterized all B. parapertussis isolates collected in the 2016-2022 period by the French National Reference Centre for Whooping Cough.Results. Microbiological analyses reveal that 13 of 18 bacterial isolates collected in 2022 produce the vaccine antigen pertactin, whereas none of the 22 isolates collected in the 2016-2021 period did.Conclusion. We hypothesize a re-introduction of B. parapertussis from regions of the world where whole-cell vaccines are still in use.
摘要：
在2022年3月至10月之间，通过qPCR检测副咳博德特氏菌的高峰，在法国观察到实时PCR。假设/差距声明。这个高峰是由于以前循环血统的复兴还是重新引入该国，目前尚不清楚。Objective.这项研究的目的是了解2022年在法国观察到的B.papertussis短暂增加，而自2020年COVID-19大流行开始以来，它实际上已停止报告。方法。我们分析了来自法国两个最大的百日咳诊断门诊实验室的实时PCR（qPCR）数据，并表征了法国国家百日咳参考中心在2016-2022年期间收集的所有百日咳杆菌分离株。结果。微生物学分析显示，2022年收集的18种细菌分离株中有13种产生疫苗抗原pertactin，而在2016-2021年收集的22个分离株中,没有一个分离株这样做.结论。我们假设从世界上仍在使用全细胞疫苗的地区重新引入了副百日咳杆菌。
