PDF(Pubmed)
Abstract：
UNASSIGNED: STAT1a is an essential signal transduction protein involved in the interferon pathway, playing a vital role in IFN-alpha/beta and gamma signaling. Limited information is available about the STAT protein in fish, particularly in Indian major carps (IMC). This study aimed to identify and characterize the STAT1a protein in Labeo rohita (LrSTAT1a).
UNASSIGNED: The full-length CDS of LrSTAT1a transcript was identified and sequenced. Phylogenetic analyses were performed based on the nucleotide sequences. The in-vivo immune stimulant poly I: C was used to treat various tissues, and the expression of LrSTAT1a was determined using quantitative real-time polymerase chain reaction (qRT-PCR). A 3D model of the STAT1a protein was generated using close structure homologs available in the database and checked using molecular dynamics (MD) simulations.
UNASSIGNED: The full-length CDS of Labeo rohita STAT1a (LrSTAT1a) transcript consisted of 3238 bp that encoded a polypeptide of 721 amino acids sequence was identified. Phylogenetic analyses were performed based on the nucleotide sequences. Based on our findings, other vertebrates share a high degree of conservation with STAT1a. Additionally, we report that the in vivo immune stimulant poly I: C treatment of various tissues resulted in the expression of LrSTAT1a as determined by quantitative real-time polymerase chain reaction (qRT-PCR). In the current investigation, treatment with poly I: C dramatically increased the expression of LrSTAT1a in nearly every organ and tissue, with the brain, muscle, kidney, and intestine showing the highest levels of expression compared to the control. We made a 3D model of the STAT1a protein by using close structure homologs that were already available in the database. The model was then checked using molecular dynamics (MD) simulations. Consistent with previous research, the MD study highlighted the significance of the STAT1a protein, which is responsible for Src homology 2 (SH2) recognition. An important H-bonding that successfully retains SH2 inside the STAT1a binding cavity was determined to be formed by the conserved residues SER107, GLN530, SER583, LYS584, MET103, and ALA106.
UNASSIGNED: This study provides molecular insights into the STAT1a protein in Rohu (Labeo rohita) and highlights the potential role of STAT1a in the innate immune response in fish. The high degree of conservation of STAT1a among other vertebrates suggests its crucial role in the immune response. The in-vivo immune stimulation results indicate that STAT1a is involved in the immune response in various tissues, with the brain, muscle, kidney, and intestine being the most responsive. The 3D model and MD study provide further evidence of the significance of STAT1a in the immune response, specifically in SH2 recognition. Further research is necessary to understand the specific mechanisms involved in the IFN pathway and the role of STAT1a in the immune response of IMC.
UNASSIGNED: The full-length CDS of LrSTAT1a transcript was identified and sequenced. Phylogenetic analyses were performed based on the nucleotide sequences. The in-vivo immune stimulant poly I: C was used to treat various tissues, and the expression of LrSTAT1a was determined using quantitative real-time polymerase chain reaction (qRT-PCR). A 3D model of the STAT1a protein was generated using close structure homologs available in the database and checked using molecular dynamics (MD) simulations.
UNASSIGNED: The full-length CDS of Labeo rohita STAT1a (LrSTAT1a) transcript consisted of 3238 bp that encoded a polypeptide of 721 amino acids sequence was identified. Phylogenetic analyses were performed based on the nucleotide sequences. Based on our findings, other vertebrates share a high degree of conservation with STAT1a. Additionally, we report that the in vivo immune stimulant poly I: C treatment of various tissues resulted in the expression of LrSTAT1a as determined by quantitative real-time polymerase chain reaction (qRT-PCR). In the current investigation, treatment with poly I: C dramatically increased the expression of LrSTAT1a in nearly every organ and tissue, with the brain, muscle, kidney, and intestine showing the highest levels of expression compared to the control. We made a 3D model of the STAT1a protein by using close structure homologs that were already available in the database. The model was then checked using molecular dynamics (MD) simulations. Consistent with previous research, the MD study highlighted the significance of the STAT1a protein, which is responsible for Src homology 2 (SH2) recognition. An important H-bonding that successfully retains SH2 inside the STAT1a binding cavity was determined to be formed by the conserved residues SER107, GLN530, SER583, LYS584, MET103, and ALA106.
UNASSIGNED: This study provides molecular insights into the STAT1a protein in Rohu (Labeo rohita) and highlights the potential role of STAT1a in the innate immune response in fish. The high degree of conservation of STAT1a among other vertebrates suggests its crucial role in the immune response. The in-vivo immune stimulation results indicate that STAT1a is involved in the immune response in various tissues, with the brain, muscle, kidney, and intestine being the most responsive. The 3D model and MD study provide further evidence of the significance of STAT1a in the immune response, specifically in SH2 recognition. Further research is necessary to understand the specific mechanisms involved in the IFN pathway and the role of STAT1a in the immune response of IMC.
摘要：
■STAT1a是参与干扰素途径的必需信号转导蛋白，在IFN-α/β和γ信号中起着至关重要的作用。关于鱼类中STAT蛋白的信息有限，特别是在印度主要鲤鱼(IMC)。本研究旨在鉴定和表征Labeorohita中的STAT1a蛋白（LrSTAT1a）。
■对LrSTAT1a转录本的全长CDS进行鉴定和测序。基于核苷酸序列进行系统发育分析。体内免疫刺激剂聚I：C用于治疗各种组织，采用实时定量聚合酶链反应(qRT-PCR)检测LrSTAT1a的表达。使用数据库中可获得的紧密结构同系物生成STAT1a蛋白的3D模型，并使用分子动力学（MD）模拟进行检查。
■LabeorohitaSTAT1a（LrSTAT1a）转录本的全长CDS由3238bp组成，编码721个氨基酸序列的多肽。基于核苷酸序列进行系统发育分析。根据我们的发现,其他脊椎动物与STAT1a具有高度的保守性。此外,我们报道，通过定量实时聚合酶链反应（qRT-PCR）测定，体内免疫刺激剂polyI：C处理各种组织导致LrSTAT1a的表达。在目前的调查中,用polyI:C处理显著增加了LrSTAT1a在几乎每个器官和组织中的表达，用大脑，肌肉，肾,和肠显示与对照相比的最高表达水平。我们通过使用已经在数据库中获得的紧密结构同源物,制作了STAT1a蛋白的3D模型。然后使用分子动力学(MD)模拟检查模型。与以前的研究一致，MD研究强调了STAT1a蛋白的重要性，其负责Src同源性2(SH2)识别。成功地将SH2保留在STAT1a结合腔内的重要H键被确定为由保守残基SER107、GLN530、SER583、LYS584、MET103和ALA106形成。
■这项研究提供了对Rohu（Labeorohita）中STAT1a蛋白的分子见解，并强调了STAT1a在鱼类先天免疫反应中的潜在作用。在其他脊椎动物中，STAT1a的高度保守性表明其在免疫反应中的关键作用。体内免疫刺激结果表明，STAT1a参与各种组织的免疫反应，用大脑，肌肉，肾,肠道反应最灵敏。3D模型和MD研究为STAT1a在免疫应答中的意义提供了进一步的证据，特别是在SH2识别。进一步的研讨须要懂得IFN通路所触及的具体机制和STAT1a在IMC免疫反响中的感化。
■对LrSTAT1a转录本的全长CDS进行鉴定和测序。基于核苷酸序列进行系统发育分析。体内免疫刺激剂聚I：C用于治疗各种组织，采用实时定量聚合酶链反应(qRT-PCR)检测LrSTAT1a的表达。使用数据库中可获得的紧密结构同系物生成STAT1a蛋白的3D模型，并使用分子动力学（MD）模拟进行检查。
■LabeorohitaSTAT1a（LrSTAT1a）转录本的全长CDS由3238bp组成，编码721个氨基酸序列的多肽。基于核苷酸序列进行系统发育分析。根据我们的发现,其他脊椎动物与STAT1a具有高度的保守性。此外,我们报道，通过定量实时聚合酶链反应（qRT-PCR）测定，体内免疫刺激剂polyI：C处理各种组织导致LrSTAT1a的表达。在目前的调查中,用polyI:C处理显著增加了LrSTAT1a在几乎每个器官和组织中的表达，用大脑，肌肉，肾,和肠显示与对照相比的最高表达水平。我们通过使用已经在数据库中获得的紧密结构同源物,制作了STAT1a蛋白的3D模型。然后使用分子动力学(MD)模拟检查模型。与以前的研究一致，MD研究强调了STAT1a蛋白的重要性，其负责Src同源性2(SH2)识别。成功地将SH2保留在STAT1a结合腔内的重要H键被确定为由保守残基SER107、GLN530、SER583、LYS584、MET103和ALA106形成。
■这项研究提供了对Rohu（Labeorohita）中STAT1a蛋白的分子见解，并强调了STAT1a在鱼类先天免疫反应中的潜在作用。在其他脊椎动物中，STAT1a的高度保守性表明其在免疫反应中的关键作用。体内免疫刺激结果表明，STAT1a参与各种组织的免疫反应，用大脑，肌肉，肾,肠道反应最灵敏。3D模型和MD研究为STAT1a在免疫应答中的意义提供了进一步的证据，特别是在SH2识别。进一步的研讨须要懂得IFN通路所触及的具体机制和STAT1a在IMC免疫反响中的感化。
