■本研究旨在评估尼古丁粉末对血液生理的负面影响,以及Labeorohita的生化和组织学改变。
■鱼分为四组(1-4)。鱼群2、3和4暴露于不同浓度的尼古丁,例如0.75、1.25和1.75mg/l,而第1组作为对照。为了找出尼古丁对身体生理的长期影响,我们做了一个42天的实验.实验完成后,血液学,生化化验,做了组织学检查.
■结果显示HGB显著增加,红细胞,WBC,血细胞比容,平均红细胞体积,红细胞分布宽度-SD,降钙素原,中性粒细胞,淋巴细胞,单核细胞,甘油三酯,总胆固醇,低密度脂蛋白,极低密度脂蛋白,丙氨酸转氨酶,天冬氨酸转氨酶,球蛋白,促甲状腺激素,BUN,肌酐,和血糖水平,而平均红细胞血红蛋白浓度,平均红细胞血红蛋白,RDW,血小板,高密度脂蛋白,白蛋白,总蛋白质,与对照组鱼相比,暴露鱼的T3水平显着降低(p≤0.05)。组织学改变表明,暴露于无烟尼古丁会在肝脏中引起有害和退化作用,肾,和裸露的鱼片。
■在鱼中施用尼古丁会对不同的生化和血液学参数产生不利影响,并导致暴露鱼的某些重要器官发生组织学改变。
UNASSIGNED: The present research was conducted to evaluate the negative effects of nicotine powder on the blood physiology, and biochemical and histological alterations of Labeo rohita.
UNASSIGNED: Fish were divided into four groups (1-4). Fish groups 2, 3, and 4 were exposed to different concentrations of nicotine, such as 0.75, 1.25, and 1.75 mg/l, while group 1 acted as a control. To find out the long-term impact of nicotine on body physiology, we conducted a 42-day experiment. After the completion of the experiment, hematology, biochemical assays, and histology were done.
UNASSIGNED: Results revealed a considerable increase in HGB, red blood cells, WBCs, hematocrit, mean corpuscular volume, red cell distribution width -SD, procalcitonin, neutrophils, lymphocytes, monocytes, triglycerides, total cholesterol, low-density lipoprotein, very low-density lipoprotein, alanine transaminase, aspartate aminotransferase, globulin, thyroid stimulating hormone, BUN, creatinine, and blood glucose levels, whereas mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, RDW, platelet, high-density lipoprotein, albumin, total proteins, and T3 levels were significantly (p ≤ 0.05) decreased in exposed fish as compared to control group fish. Histological alterations showed that exposure to smokeless nicotine causes deleterious and degenerative effects in the liver, kidney, and gills of exposed fish.
UNASSIGNED: Nicotine administration in fish results in adverse effects on different biochemical and hematological parameters and causes histological alterations in some vital organs of exposed fish.