Abstract：
Foliar pigmentation patterns vary among plant species and growth conditions. In this study, we utilize hyperspectral imaging to assess foliar pigmentation in the bryophyte Marchantia polymorpha under nutrient stress and identify associated genetic factors. Using singular value decomposition (SVD) for feature selection, we quantitate color variations induced by deficiencies in phosphate, nitrate, magnesium, calcium, and iron. Pseudo-colored thallus images show that disrupting MpWRKY10 causes irregular pigmentation with auronidin accumulation. Transcriptomic profiling shows that MpWRKY10 regulates phenylpropanoid pathway enzymes and R2R3-MYB transcription factors during phosphate deficiency, with MpMYB14 upregulation preceding pigment accumulation. MpWRKY10 is downregulated in older, pigmented thalli under phosphate deficiency but maintained in young thalli, where it suppresses pigmentation genes. This downregulation is absent in pigmented thalli due to aging. Comparative transcriptome analysis suggests similar WRKY and MYB roles in nutrient response and pigmentation in red-leaf lettuce, alluding to conserved genetic factors controlling foliar pigmentation patterns under nutrient deficiency.
摘要：
叶面色素沉着模式因植物种类和生长条件而异。在这项研究中,我们利用高光谱成像来评估营养胁迫下苔藓植物中的叶面色素沉着，并确定相关的遗传因素。使用奇异值分解(SVD)进行特征选择，我们定量由磷酸盐缺乏引起的颜色变化，硝酸盐,镁，钙，和铁。伪彩色thallus图像显示，破坏MpWRKY10会导致不规则的色素沉着，并伴有奥罗尼丁的积累。转录组分析显示MpWRKY10在磷酸盐缺乏过程中调节苯丙素途径酶和R2R3-MYB转录因子，在色素积累之前MpMYB14上调。MpWRKY10在老年人中下调，缺乏磷酸盐但在年轻的thalli中维持的色素thalli，它抑制色素沉着基因。由于衰老，色素沉着的thalli中没有这种下调。比较转录组分析表明，在红叶莴苣中，WRKY和MYB在营养响应和色素沉着中的作用相似，暗示在营养缺乏下控制叶面色素沉着模式的保守遗传因素。
