亚洲镰刀菌是一种破坏性的植物病原真菌,可导致小麦镰刀菌枯萎病(FHB),导致全球粮食作物的严重产量和经济损失。我们以前的研究表明,靶位点突变(K216R/E,S217P/L,或E420K/G/D)的I型肌球蛋白FaMyo5赋予了对非那普利的高抗性。这里,我们首先构建了1株敏感菌株H1S和3个点突变抗性菌株HA,HC和H1R。然后,我们在1和10μg·mL-1菲纳普利处理后,对这些F.asiaticum菌株进行了比较转录组分析。结果表明,在敏感和抗性菌株中,有2135个基因差异表达(DEGs)。DEGs编码铵转运蛋白MEP1/MEP2,硝酸还原酶,铜胺氧化酶1,4-氨基丁酸氨基转移酶,氨基酸通透酶inda1,琥珀酸半醛脱氢酶,2,3-二羟基苯甲酸脱羧酶,等。,在所有耐非那普利菌株中均显著上调。与对照组相比,经过1和10μg·mL-1的菲加普利处理后,在这些菌株中总共鉴定出1778和2097DEGs,分别。这些DEGs涉及4-氨基丁酸氨基转移酶,几丁质合成酶1,多蛋白桥接因子1,转录调节蛋白pro-1,氨基酸通透酶inda1,ATP依赖性RNA解旋酶DED1,乙酰辅酶A合成酶,肌浆网/内质网钙ATP酶2等。,经非那普利处理后,在非那普利敏感菌株中表达显著下调,而在耐药菌株中表达不显著。此外,氰化物水合酶,交配型蛋白MAT-1,假定的嘌呤核苷通透酶,质膜蛋白质yro2等.,在所有的菌株中,在非那普利处理后都显示出显著的共同下调的表达。一起来看,这项研究提供了深入的见解抗性调节机制和杀菌剂的抑制作用,这些新的注释蛋白或酶是值得发现新的杀菌剂靶标。
Fusarium asiaticum is a destructive phytopathogenic fungus that causes Fusarium head blight of wheat (FHB), leading to serious yield and economic losses to cereal crops worldwide. Our previous studies indicated that target-site mutations (K216R/E, S217P/L, or E420K/G/D) of Type I myosin FaMyo5 conferred high resistance to phenamacril. Here, we first constructed one sensitive strain H1S and three point mutation resistant strains HA, HC and H1R. Then we conducted comparative transcriptome analysis of these F. asiaticum strains after 1 and 10 μg·mL-1 phenamacril treatment. Results indicated that 2135 genes were differentially expressed (DEGs) among the sensitive and resistant strains. The DEGs encoding ammonium transporter MEP1/MEP2, nitrate reductase, copper amine oxidase 1, 4-aminobutyrate aminotransferase, amino-acid permease inda1, succinate-semialdehyde dehydrogenase, 2, 3-dihydroxybenzoic acid decarboxylase, etc., were significantly up-regulated in all the phenamacril-resistant strains. Compared to the control group, a total of 1778 and 2097 DEGs were identified in these strains after 1 and 10 μg·mL-1 phenamacril treatment, respectively. These DEGs involved in 4-aminobutyrate aminotransferase, chitin synthase 1, multiprotein-bridging factor 1, transcriptional regulatory protein pro-1, amino-acid permease inda1, ATP-dependent RNA helicase DED1, acetyl-coenzyme A synthetase, sarcoplasmic/endoplasmic reticulum calcium ATPase 2, etc., showed significantly down-regulated expression in phenamacril-sensitive strain but not in resistant strains after phenamacril treatment. In addition, cyanide hydratase, mating-type protein MAT-1, putative purine nucleoside permease, plasma membrane protein yro2, etc., showed significantly co-down-regulated expression in all the strains after phenamacril treatment. Taken together, This study provides deep insights into the resistance regulation mechanism and the inhibitory effect of fungicide phenamacril and these new annotated proteins or enzymes are worth for the discovery of new fungicide targets.