Abstract：
The engineering of plant cell cultures to produce high-value natural products is suggested to be a safe, low-cost, and environmentally friendly route to produce a wide range of chemicals. Given that the expression of heterologous biosynthetic pathways in plant tissue culture is limited by a lack of detailed protocols, the biosynthesis of high-value metabolites in plant cell culture is constrained compared with that in microbes. However, both Arabidopsis thaliana and Nicotiana benthamiana can be efficiently transformed with multigene constructs to produce high-value natural products in stable plant cell cultures. This chapter provides a detailed protocol as to how to engineer the plant cell culture as bio-factories for metabolite biosynthesis.
摘要：
植物细胞培养产生高价值天然产物的工程建议是一种安全的，低成本,和环境友好的路线，以生产各种化学品。鉴于植物组织培养中异源生物合成途径的表达受到缺乏详细方案的限制，与微生物相比，植物细胞培养物中高价值代谢物的生物合成受到限制。然而,拟南芥和本氏烟草都可以用多基因构建体有效转化，以在稳定的植物细胞培养物中产生高价值的天然产物。本章提供了有关如何将植物细胞培养物作为代谢产物生物合成的生物工厂的详细协议。
