{Reference Type}: Journal Article
{Title}: Plant Tissue Culture and Metabolite Profiling for High-Value Natural Product Synthesis.
{Author}: Zhang Y;Qin K;Fernie AR;
{Journal}: Methods Mol Biol
{Volume}: 2827
{Issue}: 0
{Year}: 2024
暂无{DOI}: 10.1007/978-1-0716-3954-2_27
{Abstract}: The engineering of plant cell cultures to produce high-value natural products is suggested to be a safe, low-cost, and environmentally friendly route to produce a wide range of chemicals. Given that the expression of heterologous biosynthetic pathways in plant tissue culture is limited by a lack of detailed protocols, the biosynthesis of high-value metabolites in plant cell culture is constrained compared with that in microbes. However, both Arabidopsis thaliana and Nicotiana benthamiana can be efficiently transformed with multigene constructs to produce high-value natural products in stable plant cell cultures. This chapter provides a detailed protocol as to how to engineer the plant cell culture as bio-factories for metabolite biosynthesis.