%0 Journal Article
%T Plant Tissue Culture and Metabolite Profiling for High-Value Natural Product Synthesis.
%A Zhang Y
%A Qin K
%A Fernie AR
%J Methods Mol Biol
%V 2827
%N 0
%D 2024
%M 38985285
暂无%R 10.1007/978-1-0716-3954-2_27
%X The engineering of plant cell cultures to produce high-value natural products is suggested to be a safe, low-cost, and environmentally friendly route to produce a wide range of chemicals. Given that the expression of heterologous biosynthetic pathways in plant tissue culture is limited by a lack of detailed protocols, the biosynthesis of high-value metabolites in plant cell culture is constrained compared with that in microbes. However, both Arabidopsis thaliana and Nicotiana benthamiana can be efficiently transformed with multigene constructs to produce high-value natural products in stable plant cell cultures. This chapter provides a detailed protocol as to how to engineer the plant cell culture as bio-factories for metabolite biosynthesis.