Abstract：
Indigenous microbial communities in smelting areas are crucial for maintaining fragile ecosystem functions. However, the community assembly process and their responses to polymetallic pollution are poorly understood, especially the taxa in each bin from the amplicons that contributed to the assembly process. Herein, microbial diversity, co-occurrence patterns, assembly process and the intrinsic mechanisms across contamination gradients at a typical PbZn smelting site were systematically unravelled by high-throughput sequencing. The results showed a consistent compositional profile among the indigenous communities across sampling sites, wherein genera KD4-96 from Chloroflexi and Sphingomonas from Proteobacteria emerged as the most abundant taxa. Network modularity of the high- and middle-contaminated communities at Pb and Zn smelting sites was >0.44, indicating that community populations were clustered into modules to resist high heavy metal stress. Stochastic processes dominated the community assembly, with the greatest contribution from drift (DR), which was significantly correlated with Pb, Zn, Cr and Cu contents. What\'s particular was that the DR-controlled bins were dominated by Proteobacteria (typical r-strategists), while the HoS-controlled bins were by Chloroflexi (typical K-strategists). Furthermore, the proportion of DR in the bins dominated by Sphingomonadaceae (phylum Proteobacteria) increased gradually with the increase of heavy metal contents. These discoveries provide essential insights for community control in restoring and mitigating soil degradation at PbZn smelting sites.
摘要：
冶炼地区的土著微生物群落对于维持脆弱的生态系统功能至关重要。然而,社区集会过程及其对多金属污染的反应知之甚少，特别是每个仓中的类群，这些类群来自对组装过程有贡献的扩增子。在这里，微生物多样性,共现模式，通过高通量测序系统地揭示了典型的PbZn冶炼现场的组装过程和污染梯度的内在机理。结果表明，不同采样点的土著社区之间的组成特征一致，其中来自绿氟菌的KD4-96属和来自变形杆菌的鞘氨醇单胞菌属是最丰富的分类群。铅和锌冶炼地点的高污染和中污染群落的网络模块性>0.44，表明群落种群聚集成模块以抵抗高重金属胁迫。随机过程主导了社区集会，漂移(DR)的贡献最大，与铅显著相关，Zn,Cr和Cu含量。特别的是，DR控制的垃圾箱主要由变形杆菌(典型的r策略师)，而HoS控制的垃圾箱是由Chloroflexi(典型的K战略家)控制的。此外，随着重金属含量的增加，以Sphingomonadaceae（变形杆菌）为主的垃圾箱中DR的比例逐渐增加。这些发现为社区控制在恢复和减轻PbZn冶炼地点的土壤退化提供了重要见解。
