Abstract：
Toll-like receptors (TLRs) represent a prominent category of pattern recognition receptors that have been extensively investigated for their pivotal role in combating pathogen incursions. Despite this, there has been a notable absence of comprehensive identification and exploration of the immune response associated with the TLR family genes in C. altivelis. This study successfully identified and named fourteen genes as Catlr1-1, Catlr1-2, Catlr2-1, Catlr2-2, Catlr3, Catlr5, Catlr7, Catlr8, Catlr9, Catlr13-1, Catlr13-2, Catlr18, Catlr21, and Catlr22. A series of bioinformatic analysis were performed, encompassing analysis of protein properties, examination of gene structures, evolutionary assessments, and prediction of protein tertiary structures. The expression patterns of Catlr genes were analyzed in five immune tissues: liver, spleen, kidney, gill, and intestine, in both healthy and bacterial stimulated-fish. The results showed that different tissue and different genes showed differed expression patterns after V. harveyi infection, indicating the involvement of all Catlr members in mounting immune responses following infection in various tissues. Additionally, histological evaluations of immune tissues unveiled varying levels of damage. In conclusion, this investigation into the TLR gene family offers novel information that contribute to a more profound comprehension of the immune response mechanisms in C. altivelis.
摘要：
Toll样受体（TLR）代表了模式识别受体的一个重要类别，已对其在抵抗病原体入侵中的关键作用进行了广泛研究。尽管如此,在C.altivelis中，明显缺乏与TLR家族基因相关的免疫反应的全面鉴定和探索。本研究成功鉴定并命名了14个基因：Catlr1-1，Catlr1-2，Catlr2-1，Catlr2-2，Catlr3，Catlr5，Catlr7，Catlr8，Catlr9，Catlr13-1，Catlr13-2，Catlr18，Catlr21和Catlr22。进行了一系列生物信息学分析，包括蛋白质特性的分析，检查基因结构，进化评估，和蛋白质三级结构的预测。分析了Catlr基因在五种免疫组织中的表达模式：肝脏，脾,脾肾,吉尔,和肠，在健康和细菌刺激的鱼类中。结果表明,不同组织和不同基因在哈氏弧菌感染后表现出不同的表达模式,表明所有Catlr成员参与各种组织感染后的免疫反应。此外,免疫组织的组织学评估揭示了不同程度的损伤。总之,这项对TLR基因家族的研究提供了新的信息，有助于更深入地理解C.altivelis的免疫应答机制。
