象牙壳(Babyloniaareolata)是最有前途的高品质海产品之一。然而,象牙壳在培养期间容易感染哈维氏弧菌。在这项研究中,我们调查了生化指标,PBS对照组(PC)和感染组(A3)在24小时后1×109CFU/mLV.harveyi的象牙壳肝胰腺的组织学变化和转录组反应。结果表明,与PC组相比,生化指标,包括丙二醛(MDA),活性氧(ROS),酸性磷酸酶(ACP),和Caspase3(Casp-3)在A3组感染弧菌24h后显著升高(p<0.05)。A3组肝胰腺损伤严重,组织的柱状上皮细胞增大,消化细胞的空间增加了,出现了空泡腔。在感染组中总共获得了95,581个单基因,并鉴定了2,949个(1,787个上调和1,162个下调)差异表达基因(DEGs)。GO和KEGG富集分析表明,DEGs主要富集在免疫系统过程中(GO:0002376),抗氧化活性(GO:0016209),溶酶体(ko04142),Toll和IMD信号通路(ko04624),和其他人。这些生物学功能和途径与免疫和炎症反应以及细胞凋亡有关。随机选择12个DEGs进行实时定量PCR(RT-qPCR)验证,这些DEGs的表达谱与转录组数据一致,确认转录组结果的准确性和可靠性。总之,V.哈维伊象牙壳感染诱导氧化应激,导致严重的肝胰腺损伤,刺激谷胱甘肽的产生以中和过量的ROS,和抗菌肽的生产,以抵消细菌感染的有害影响,反过来改变免疫和炎症反应,最终导致细胞凋亡。本研究为探索哈维氏弧菌感染后的免疫调节机制提供了有价值的信息,并为预防哈维氏弧菌感染提供了分子依据。
The ivory shell (Babylonia areolata) is one of the most promising high quality marine products. However, ivory shell is susceptible to Vibrio harveyi infection during the culture period. In this study, we investigated the biochemical indicators, histological changes and transcriptomic response in the hepatopancreas of ivory shells from the PBS control group (PC) and infection group (A3) with 1 × 109 CFU/mL V. harveyi after 24 h. Results showed that compared to the PC group, biochemical indicators, including malondialdehyde (MDA), reactive oxygen species (ROS), acid phosphatase (ACP), and Caspase 3 (Casp-3) were significantly increased (p < 0.05) in A3 group after V. harveyi infection for 24 h. Compared with the PC group, the hepatopancreas of A3 group were seriously damaged, the columnar epithelial cells of the tissue were enlarged, the space of digestive cells was increased, and vacuolar cavities appeared. A total of 95,581 unigenes were obtained and 2949 (1787 up-regulated and 1162 down-regulated) differential expressed genes (DEGs) were identified in the infecting group. GO and KEGG enrichment analysis showed that DEGs were mainly enriched in immune system process (GO:0002376), antioxidant activity (GO:0016209), lysosome (ko04142), Toll and IMD signaling pathway (ko04624), and others. These biological functions and pathways are associated with immune and inflammatory responses and apoptosis. Twelve DEGs were randomly selected for real-time quantitative PCR (RT-qPCR) validation, and the expression profiles of these DEGs were consistent with the transcriptome data, confirming the accuracy and reliability of the transcriptome results. In summary, V. harveyi infection of ivory shells inducing oxidative stress, leading to severe hepatopancreatic damage, stimulating glutathione production to neutralize excessive ROS, and antimicrobial peptides production to counteract the deleterious effects of bacterial infection, which in turn modifying the immune and inflammatory response, ultimately resulting in apoptosis. This study provided valuable information to explore the immune regulation mechanism after V. harveyi infection and provides molecular basis to support the prevention of V. harveyi infection.