■溶藻弧菌是革兰氏阴性,属于弧菌科的杆状细菌,水产养殖动物中常见的病原体,然而,关于其对锯缘青蟹(泥蟹)影响的研究有限。在这项研究中,在香港水产养殖场爆发疾病期间,我们从死泥蟹中分离出溶藻弧菌。在夏季造成高达70%的死亡率。
■实验感染和组织病理学研究溶藻弧菌SWS在锯缘链球菌中的致病性,并验证Koch的假设。全面的全基因组分析和系统发育分析抗菌素敏感性测试,和生化表征也进行了。
■我们的发现表明,溶藻弧菌SWS在锯缘链球菌中引起高死亡率(75%),感染个体表现出不活动,食欲不振,褪色和变暗的肝胰腺,ill,爪部肌肉不透明.组织病理学分析显示肝胰腺组织损伤和变性,ill,和爪肌提示溶藻弧菌SWS感染的直接和间接影响。
■这项研究提供了作为锯缘链球菌水产养殖中新兴病原体的溶藻弧菌SWS的全面表征。我们的发现强调了持续监测的重要性,早期发现,并制定针对性的疾病管理策略,以减轻泥蟹水产养殖中弧菌病暴发的经济影响。
UNASSIGNED: Vibrio alginolyticus is a Gram-negative, rod-shaped bacterium belonging to the family of Vibrionaceae, a common pathogen in aquaculture animals, However, studies on its impact on Scylla serrata (mud crabs) are limited. In this study, we isolated V. alginolyticus SWS from dead mud crab during a disease outbreak in a Hong Kong aquaculture farm, which caused up to 70% mortality during summer.
UNASSIGNED: Experimental infection and histopathology were used to investigate the pathogenicity of V. alginolyticus SWS in S. serrata and validate Koch\'s postulates. Comprehensive whole-genome analysis and phylogenetic analysis antimicrobial susceptibility testing, and biochemical characterization were also performed.
UNASSIGNED: Our findings showed that V. alginolyticus SWS caused high mortality (75%) in S. serrata with infected individuals exhibiting inactivity, loss of appetite, decolored and darkened hepatopancreas, gills, and opaque muscle in the claw. Histopathological analysis revealed tissue damage and degeneration in the hepatopancreas, gills, and claw muscle suggesting direct and indirect impacts of V. alginolyticus SWS infection.
UNASSIGNED: This study provides a comprehensive characterization of V. alginolyticus SWS as an emerging pathogen in S. serrata aquaculture. Our findings underscore the importance of ongoing surveillance, early detection, and the development of targeted disease management strategies to mitigate the economic impact of vibriosis outbreaks in mud crab aquaculture.