PDF(Pubmed)
Abstract：
Vertical transmission, the transfer of pathogens across generations, is a critical mechanism for the persistence of plant viruses. The transmission mechanisms are diverse, involving direct invasion through the suspensor and virus entry into developing gametes before achieving symplastic isolation. Despite the progress in understanding vertical virus transmission, the environmental factors influencing this process remain largely unexplored. We investigated the complex interplay between vertical transmission of plant viruses and pollination dynamics, focusing on common bean (Phaseolus vulgaris). The intricate relationship between plants and pollinators, especially bees, is essential for global ecosystems and crop productivity. We explored the impact of virus infection on seed transmission rates, with a particular emphasis on bean common mosaic virus (BCMV), bean common mosaic necrosis virus (BCMNV), and cucumber mosaic virus (CMV). Under controlled growth conditions, BCMNV exhibited the highest seed transmission rate, followed by BCMV and CMV. Notably, in the field, bee-pollinated BCMV-infected plants showed a reduced transmission rate compared to self-pollinated plants. This highlights the influence of pollinators on virus transmission dynamics. The findings demonstrate the virus-specific nature of seed transmission and underscore the importance of considering environmental factors, such as pollination, in understanding and managing plant virus spread.
摘要：
垂直传动,病原体跨代转移，是植物病毒持续存在的关键机制。传播机制多种多样,在实现共生体分离之前，涉及通过悬液直接入侵和病毒进入发育中的配子。尽管在理解病毒垂直传播方面取得了进展，影响这一过程的环境因素在很大程度上仍未被探索。我们调查了植物病毒垂直传播与授粉动力学之间的复杂相互作用，专注于普通豆（菜豆）。植物和传粉者之间错综复杂的关系，尤其是蜜蜂,对全球生态系统和作物生产力至关重要。我们探讨了病毒感染对种子传播率的影响，特别强调豆类普通花叶病毒(BCMV)，豆普通花叶坏死病毒（BCMNV），和黄瓜花叶病毒（CMV）。在受控生长条件下，BCMNV表现出最高的种子传输速率，其次是BCMV和CMV。值得注意的是,在野外,与自花授粉的植物相比，蜜蜂授粉的BCMV感染的植物的传播率降低。这突出了传粉者对病毒传播动力学的影响。研究结果证明了种子传播的病毒特异性，并强调了考虑环境因素的重要性，比如授粉,了解和管理植物病毒传播。
