转录因子的NAC家族包括无顶端分生组织(NAM),拟南芥转录激活因子1/2(ATAF1/2),和杯状子叶(CUC2)蛋白,这是植物特有的,大大有助于他们适应环境挑战。在本研究中,我们观察到PvNAC52蛋白主要在细胞膜上表达,细胞质,和核。PvNAC52在拟南芥中的过表达增强了植物对盐的抗逆性,碱,渗透,和ABA强调。PvNAC52显著(p<0.05)降低了细胞膜的氧化损伤程度,脯氨酸含量,和植物水分损失通过增加MSD1,FSD1,CSD1,POD的表达,PRX69,CAT,P5CS2此外,与非生物应激反应相关基因的表达,如SOS1,P5S1,RD29A,NCED3,ABIs,LEAs,和DREB,PvNAC52过表达增强。酵母单杂交分析表明,PvNAC52特异性结合顺式作用元件ABRE(脱落酸反应元件,ACGTG)在启动子内。这进一步表明,PvNAC52通过鉴定核心序列负责非生物应激反应基因的转录调节,ACGTG.这些发现为进一步分析普通豆中PvNAC52的靶向顺式作用元件和下游基因提供了理论基础。
The NAC family of transcription factors includes no apical meristem (NAM), Arabidopsis thaliana transcription activator 1/2 (ATAF1/2), and cup-shaped cotyledon (CUC2) proteins, which are unique to plants, contributing significantly to their adaptation to environmental challenges. In the present study, we observed that the PvNAC52 protein is predominantly expressed in the cell membrane, cytoplasm, and nucleus. Overexpression of PvNAC52 in Arabidopsis strengthened plant resilience to salt, alkali, osmotic, and ABA stresses. PvNAC52 significantly (p < 0.05) reduced the degree of oxidative damage to cell membranes, proline content, and plant water loss by increasing the expression of MSD1, FSD1, CSD1, POD, PRX69, CAT, and P5CS2. Moreover, the expression of genes associated with abiotic stress responses, such as SOS1, P5S1, RD29A, NCED3, ABIs, LEAs, and DREBs, was enhanced by PvNAC52 overexpression. A yeast one-hybrid assay showed that PvNAC52 specifically binds to the cis-acting elements ABRE (abscisic acid-responsive elements, ACGTG) within the promoter. This further suggests that PvNAC52 is responsible for the transcriptional modulation of abiotic stress response genes by identifying the core sequence, ACGTG. These findings provide a theoretical foundation for the further analysis of the targeted cis-acting elements and genes downstream of PvNAC52 in the common bean.