辣椒(CapsicumannuumL.)是在印度次大陆种植的重要蔬菜作物,在田间条件下容易发生病毒感染。在实地调查期间,来自辣椒植物的叶片样品显示出由黄瓜花叶病毒(CMV)引起的疾病的典型症状,例如轻度花叶病毒,收集斑点和叶片变形。DAC-ELISA分析证实100个样本中有71个存在CMV,表明其在该地区的广泛流行。五个CMV分离株,名为Gu1,Gu2,BA,Ho,将Sal机械接种到黄瓜和烟草上,研究其毒力。接种的植物表现出CMV的特征性症状,例如褪绿斑点,然后是轻度马赛克和叶片变形。五个CMV分离株的完整基因组被扩增,克隆,并测序,揭示具有3358、3045和2220个核苷酸的RNA1、RNA2和RNA3序列,分别。系统发育分析将分离株分类为属于CMV-IB亚组,将它们与IA和II亚型CMV分离株区分开。重组分析显示这些分离株的所有三个RNA片段中的种内和种间重组。在13种寄主植物的CMV-宿主相互作用过程中,使用了蛋白质-蛋白质对接方法来破译马赛克症状产生的机制。分析表明,马赛克症状的产生可能是由于CMV的外壳蛋白(CP)与叶绿体铁氧还蛋白之间的相互作用。Further,通过机械树液接种在13株CMV寄主植物中验证了计算机预测。十二株寄主植物产生了全身性症状。,褪绿斑点,褪绿环斑,褪绿局部病变,马赛克和温和的马赛克和一个寄主植物,茄子产生马赛克,随后出现鞋带症状。
■在线版本包含补充材料,可在10.1007/s13205-023-03777-8获得。
Chilli (Capsicum annuum L.) is an important vegetable crop grown in the Indian sub-continent and is prone to viral infections under field conditions. During the field survey, leaf samples from chilli plants showing typical symptoms of disease caused by cucumber mosaic virus (CMV) such as mild mosaic, mottling and leaf distortion were collected. DAC-ELISA analysis confirmed the presence of CMV in 71 out of 100 samples, indicating its widespread prevalence in the region. Five CMV isolates, named Gu1, Gu2, BA, Ho, and Sal were mechanically inoculated onto cucumber and Nicotiana glutinosa plants to study their virulence. Inoculated plants expressed the characteristic symptoms of CMV such as chlorotic spots followed by mild mosaic and leaf distortion. Complete genomes of the five CMV isolates were amplified, cloned, and sequenced, revealing RNA1, RNA2, and RNA3 sequences with 3358, 3045, and 2220 nucleotides, respectively. Phylogenetic analysis classified the isolates as belonging to the CMV-IB subgroup, distinguishing them from subgroup IA and II CMV isolates. Recombination analysis showed intra and interspecific recombination in all the three RNA segments of these isolates. In silico protein-protein docking approach was used to decipher the mechanism behind the production of mosaic symptoms during the CMV-host interaction in 13 host plants. Analysis revealed that the production of mosaic symptoms could be due to the interaction between the coat protein (CP) of CMV and chloroplast ferredoxin proteins. Further, in silico prediction was validated in 13 host plants of CMV by mechanical sap inoculation. Twelve host plants produced systemic symptoms viz., chlorotic spot, chlorotic ringspot, chlorotic local lesion, mosaic and mild mosaic and one host plant, Solanum lycopersicum produced mosaic followed by shoestring symptoms.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s13205-023-03777-8.