PDF(Pubmed)
Abstract：
The growth of Lactobacillus plantarum, a member of the Lactobacillus genus, which plays a crucial role in the bacterial microbiome of the gut, is significantly influenced by manganese ions. They can be safely delivered to the intestines by exploiting the chelating abilities of lactoferrin. The aim of this work was to encapsulate lactoferrin saturated with manganese ions (MnLf) in a system based on the Eudragit® RS polymer to protect protein from degradation and manganese release in the gastric environment. The entrapment efficiency was satisfactory, reaching about 95%, and most importantly, manganese ions were not released during microparticles (MPs) formation. The release profile of the protein from the freshly prepared MPs was sustained, with less than 15% of the protein released within the first hour. To achieve similar protein release efficiency, freeze-drying was carried out in the presence of 10% (w/v) mannitol as a cryoprotectant for MPs frozen at -20 °C. MPs with encapsulated MnLf exhibited prebiotic activity towards Lactobacillus plantarum. More importantly, the presence of equivalent levels of manganese ions in free form in the medium, as well as chelating by lactoferrin encapsulated in MPs, had a similar impact on stimulating bacterial growth. This indicates that the bioavailability of manganese ions in our prepared system is very good.
摘要：
植物乳杆菌的生长,乳杆菌属的成员，在肠道的细菌微生物群中起着至关重要的作用，受到锰离子的显著影响。通过利用乳铁蛋白的螯合能力,可以将它们安全地递送至肠。这项工作的目的是将锰离子饱和的乳铁蛋白（MnLf）封装在基于Eudragit®RS聚合物的系统中，以保护蛋白质在胃环境中免受降解和锰释放。截留效率令人满意，达到约95%,最重要的是,锰离子在微粒（MPs）形成过程中没有释放。蛋白质从新鲜制备的MP的释放曲线是持续的，在第一个小时内释放不到15%的蛋白质。为了达到相似的蛋白质释放效率，冷冻干燥在10%(w/v)甘露醇作为冷冻保护剂存在下进行,用于在-20°C冷冻的MPs。具有包封的MnLf的MPs对植物乳杆菌表现出益生元活性。更重要的是,在培养基中存在等量的自由形式的锰离子，以及被包裹在MPs中的乳铁蛋白螯合，对刺激细菌生长也有类似的影响。这表明我们制备的体系中锰离子的生物利用度非常好。
