这项研究描述了来自稻草(RS)和维管水生杂草如香豆蔻(TA)和小脑(CD)的半纤维素自水解产物(HAHs)的提取和表征。它进一步探讨了它们维持选定乳酸菌增殖的能力(即,益生元活性)从牛奶样品中分离。从RS分离HAH,采用TA和CD热水提取(HWE)法,TA,和CD生物质产生6.8、4.99和2.98%的HAH,对应的半纤维素提取效率为26.15±0.8%,23.76±0.6%,和18.62±0.4%。通过HPLC对HAH浓缩物的化学表征表明,它们包含半乳糖,阿拉伯糖,木糖和葡萄糖。RS的总酚含量,TA和CD衍生的HAH浓缩物为37.53、56.78和48.08mgGAE/g。五种乳酸菌(LAB)分离物Q1B,Q2A,Q3B,选择用于益生元活性测定的G1C和G2B产生了混合反应,对于Q2A,RS-HAH的生长最高,而对于Q3B,TA-HAH的生长最少。Further,分离株Q2A,Q3B,G1C,G2B,表现出最高的增长表现,通过MALDI-TOF和16SrRNA测序鉴定为短乳杆菌。所有测试的LAB分离株在粗HAH制剂中均显示出双生生长,以最大程度地利用碳源进行增殖。这表明所选择的LAB分离物是半纤维素糖的有效降解剂。这为木质纤维素生物质的有价化以产生益生元半纤维素自水解产物铺平了道路,并因此通过提高资源效率来增强环境可持续性。
This study describes the extraction and characterization of the hemicellulosic autohydrolysates (HAHs) derived from rice straw (RS) and vascular aquatic weeds like Typha angustifolia (TA) and Ceretophyllum demersum (CD). It further explores their capacity to sustain the proliferation of selected lactic acid bacteria (i.e., prebiotic activity) isolated from milk samples. To fractionate HAH from RS, TA and CD hot water extraction (HWE) method was used and RS, TA, and CD biomasses yielded 6.8, 4.99 and 2.98% of HAH corresponding to the hemicellulose extraction efficiencies of 26.15 ± 0.8%, 23.76 ± 0.6%, and 18.62 ± 0.4% respectively. The chemical characterization of HAH concentrates through HPLC showed that they comprised galactose, arabinose, xylose and glucose. The total phenol content of the RS, TA and CD-derived HAH concentrates were 37.53, 56.78 and 48.08 mg GAE/g. The five lactic acid bacteria (LAB) isolates Q1B, Q2A, Q3B, G1C and G2B selected for prebiotic activity assays generated mixed responses with the highest growth in RS-HAH for Q2A and the least in TA-HAH for Q3B. Further, the isolates Q2A, Q3B, G1C, and G2B, which showed the highest growth performance, were identified through MALDI-TOF and 16S rRNA sequencing as Lactobacillus brevis. All the tested LAB isolates showed diauxic growth in crude HAH preparations to maximize the utilization of carbon resources for their proliferation. This suggests that the selected LAB isolates are efficient degraders of hemicellulosic sugars. This paves the way for the valorization of lignocellulosic biomass to produce prebiotic hemicellulosic autohydrolysate and consequently enhances environmental sustainability by improving resource efficiency.