■纳米疫苗已成为一种有前途的疫苗接种策略,表现出它们递送抗原和佐剂以引发特异性免疫应答的能力。尽管有这种潜力,优化纳米疫苗的设计和交付仍然是一个挑战。
■在这项研究中,我们设计了一种树枝状介孔二氧化硅基纳米载体,包裹在含有二价锰离子和单宁酸(MSN@MT)的金属-酚网络(MPN)层中。这种纳米载体是为抗原负载量身定做的,作为纳米疫苗,旨在激活树突状细胞(DC)中干扰素基因的环状GMP-AMP合酶刺激物(cGAS-STING)途径。我们的实验方法包括细胞试验和小鼠免疫接种,可以全面评估纳米疫苗对DC活化的影响及其对抗原特异性T细胞反应产生的影响。
■MSN@MT与对照组相比,小鼠的体液和细胞免疫反应显着增强。这凸显了MSN@MT在DCs中有效触发cGAS-STING途径的潜力,导致强大的免疫反应。
■我们的研究介绍了MSN@MT,一种结合二价锰离子和单宁酸的独特纳米载体,展示了其通过激活DCs中的cGAS-STING途径来放大免疫反应的卓越能力。这一创新标志着改进纳米疫苗设计以实现有效免疫激活的大步迈进。
UNASSIGNED: Nanovaccines have emerged as a promising vaccination strategy, exhibiting their capacity to deliver antigens and adjuvants to elicit specific immune responses. Despite this potential, optimizing the design and delivery of nanovaccines remains a challenge.
UNASSIGNED: In this study, we engineered a dendritic mesoporous silica-based nanocarrier enveloped in a metal-phenolic network (MPN) layer containing divalent manganese ions and tannic acid (MSN@MT). This nanocarrier was tailored for antigen loading to serve as a nanovaccine, aiming to activate the cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS-STING) pathway in dendritic cells (DCs). Our experimental approach encompassed both cellular assays and mouse immunizations, allowing a comprehensive evaluation of the nanovaccine\'s impact on DC activation and its influence on the generation of antigen-specific T-cell responses.
UNASSIGNED: MSN@MT demonstrated a remarkable enhancement in humoral and cellular immune responses in mice compared to control groups. This highlights the potential of MSN@MT to effectively trigger the cGAS-STING pathway in DCs, resulting in robust immune responses.
UNASSIGNED: Our study introduces MSN@MT, a unique nanocarrier incorporating divalent manganese ions and tannic acid, showcasing its exceptional ability to amplify immune responses by activating the cGAS-STING pathway in DCs. This innovation signifies a stride in refining nanovaccine design for potent immune activation.