从郴州采集的银叶中分离出HUAS3-15T菌株,湖南省,公关中国。该菌株的主要脂肪酸(>5.0%)是anteiso-C15:0,C16:0,C18:1ω9c,iso-C16:0,求和特征5(C18:2ω6,9c/C18:0ante),iso-C15:0和anteiso-C17:0。MK-9(H6),MK-9(H8)和MK-9(H4)被检测为呼吸醌。诊断细胞壁二氨基酸是内消旋二氨基庚二酸。半乳糖,葡萄糖和核糖也存在于细胞壁中。主要极性脂质由二磷脂酰甘油组成,磷脂酰乙醇胺,磷脂酰肌醇甘露糖苷和未鉴定的磷脂。基因组序列的DNAG+C含量,由8860963bp组成,为72.4mol%。基于16SrRNA基因序列的blast分析表明,该菌株属于北孢菌属,具有99.37、99.03、98.95、98.68和98.67%的序列相似性,天蚕DSM44826T,KitasatosporaxanthocidicaNBRC13469T,北孢菌NRRLB-2218T和北孢菌IFO15206T,分别。基于16SrRNA基因和全基因组序列的系统发育树表明,菌株HUAS3-15T与K.aureofaciensATCC10762T形成了一个支持良好的簇。通过HUAS3-15T菌株和K之间的平均核苷酸同一性(ANIb/m)和数字DNA-DNA杂交分析进行进一步的基因组表征。aurerosofaciensATCC10762T的值分别为90.62/92.55%和45.3%,分别,低于95-96%的ANI阈值和70.0%的临界值,用作细菌中物种划分的指导值。此外,菌株与其系统基因组邻居在生理(例如唯一碳源生长)和化学分类学(例如细胞脂肪组成)特征方面的差异进一步支持了这一结论。因此,我们得出的结论是,菌株HUAS3-15T代表了北孢菌属的一种新物种,其名称为Kitasatosporacathayaesp。11月。是提议的。菌株类型为HUAS3-15T(=MCCC1K08542T=JCM36274T)。
Strain HUAS 3-15T was isolated from the leaves of Cathaya argyrophylla collected from Chenzhou, Hunan Province, PR China. The main fatty acids (>5.0 %) of the strain were anteiso-C15 : 0, C16 : 0, C18 : 1 ω9c, iso-C16 : 0, summed feature 5 (C18 : 2 ω6,9c/C18 : 0 ante), iso-C15 : 0 and anteiso-C17 : 0. MK-9(H6), MK-9(H8) and MK-9(H4) were detected as respiratory quinones. The diagnostic cell-wall diamino acid was meso-diaminopimelic acid. Galactose, glucose and ribose were also present in the cell wall. The major polar lipids consisted of diphosphatidylglycerol, phosphatidyl ethanolamine, phosphatidylinositol mannosides and unidentified phospholipids. The DNA G+C content of the genome sequence, consisting of 8 860 963 bp, is 72.4 mol%. blast analysis based on 16S rRNA gene sequences revealed that the strain belongs to the genus Kitasatospora, with 99.37, 99.03, 98.95, 98.68 and 98.67 % sequence similarity to Kitasatospora aureofaciens ATCC 10762T, Kitasatospora viridis DSM 44826T, Kitasatospora xanthocidica NBRC 13469T, Kitasatospora aburaviensis NRRL B-2218T and Kitasatospora kifunensis IFO 15206T, respectively. Phylogenetic trees based on 16S rRNA gene and whole-genome sequences demonstrated that strain HUAS 3-15T formed a well-supported cluster with K. aureofaciens ATCC 10762T. Further genomic characterization through average nucleotide identity (ANIb/m) and digital DNA-DNA hybridization analysis between strain HUAS 3-15T and K. aureofaciens ATCC 10762T showed values of 90.62/92.55 % and 45.3 %, respectively, lower than the 95-96 % ANI threshold and 70.0 % cutoff used as guideline values for species delineation in bacteria. Furthermore, the differences between the strain and its phylogenomic neighbour in terms of physiological (e.g. sole carbon source growth) and chemotaxonomic (e.g. cellular fatty composition) characteristics further supported this conclusion. Consequently, we concluded that strain HUAS 3-15T represents a novel species of the genus Kitasatospora, for which the name Kitasatospora cathayae sp. nov. is proposed. The type strain is HUAS 3-15T (=MCCC 1K08542T=JCM 36274T).