PDF(Pubmed)
Abstract：
Volatile oil serves as a traditional antipyretic component of Bupleuri Radix. Bupleurum marginatum var. stenophyllum (Wolff) Shan et Y. Li belongs to the genus Bupleurum and is distinguished for its high level of saikosaponins and volatile oils; nonetheless, prevailing evidence remains inconclusive regarding its viability as an alternative resource of other official species. This study aims to systematically compare the volatile oil components of both dried and fresh roots of B. marginatum var. stenophyllum and the four legally available Bupleurum species across their chemical, molecular, bionics, and anatomical structures. A total of 962 compounds were determined via GC-MS from the dried roots; B. marginatum var. stenophyllum showed the greatest differences from other species in terms of hydrocarbons, esters, and ketones, which was consistent with the results of fresh roots and the e-nose analysis. A large number of DEGs were identified from the key enzyme family of the monoterpene synthesis pathway in B. marginatum var. stenophyllum via transcriptome analysis. The microscopic observation results, using different staining methods, further showed the distinctive high proportion of phloem in B. marginatum var. stenophyllum, the structure which produces volatile oils. Together, these pieces of evidence hold substantial significance in guiding the judicious development and utilization of Bupleurum genus resources.
摘要：
挥发油是柴胡的传统解热成分。边缘柴胡。stenophylum（Wolff）ShanetY.Li属于柴胡属，以其高水平的柴胡皂苷和挥发油而著称；尽管如此，关于它作为其他官方物种的替代资源的可行性，目前的证据仍然没有定论。本研究旨在系统地比较边际芽孢杆菌干燥根和新鲜根的挥发油成分。stenophyllum和四种合法可用的柴胡物种，分子，仿生学,和解剖结构。通过GC-MS从干燥的根确定了总共962种化合物；边缘芽孢杆菌var。stenophyllum显示出与其他物种在碳氢化合物方面的最大差异，酯类,和酮，这与新鲜根和电子鼻分析的结果一致。从边缘芽孢杆菌中单萜合成途径的关键酶家族中鉴定出大量DEGs。通过转录组分析。显微观察结果,使用不同的染色方法，进一步显示出边缘芽孢杆菌中韧皮部的明显高比例。stophyllum,产生挥发油的结构。一起,这些证据对于指导柴胡属资源的合理开发和利用具有重要意义。
